纸质玩具中BPA和BPS化合物的迁移

Migration of BPA and BPS Compounds in Paper Toys

目的研究纸质玩具中双酚A(BPA)和双酚S(BPS)经口暴露途径下的迁移规律,并考察BPA和BPS迁移的适用性。方法采用间接酶联免疫(IC-ELISA)检测方法和Fick第二定律研究不同温度(4,37,60℃)、盐浓度(0.001,0.01,0.1 mol/L)、pH(4,7,9)条件下双酚类物质经口暴露下的迁移规律,模拟纸质玩具中BPA和BPS的迁移过程。结果双酚类物质迁移量与温度和盐离子浓度呈正相关,且在酸性和碱性条件的模拟液中溶出增加,纸质玩具中BPA和BPS经口暴露途径下的迁移规律符合Fick第二定律。正常迁移模拟条件下(37℃、盐浓度0.01 mol/L、pH=7的唾液模拟液)测得BPA和BPS的迁移系数分别为1.3×10^(-17),6.6×10^(18)cm^(2)/s。结论基于酶免疫检测方法,构建了纸质玩具中BPA和BPS的迁移模型,并成功应用于预测纸质玩具中双酚类化合物的迁移情况,根据纸质玩具中双酚类物质的迁移模拟实验,计算双酚类物质在不同条件下的扩散系数,并拟合迁移模型,预测盲样中BPA和BPS的迁移情况,与实际迁移情况对比,结果表明理论预测值与实际迁移量基本一致,此项工作对于儿童纸质玩具中双酚类物质的风险性评估具有重要意义。

The work aims to study the migration laws of bisphenol A(BPA)and bisphenol S(BPS)in paper toys by oral exposure and investigate the applicability of BPA and BPS migration.The migration laws of bisphenol compounds were studied by indirect enzyme linked immunosorbent(IC-ELISA)and Fick’s second law under the conditions of different temperature(4,37,60℃),salt concentration(0.001,0.01,0.1 mol/L),pH(4,7,9)to simulate the migration of BPA and BPS in paper toys.The migration amount was positively correlated with temperature and salt ion concentration,and the dissolution increased in the simulated solution under acidic and alkaline pH conditions.The migration laws of BPA and BPS in paper toys by oral exposure conformed to Fick’s second law.Under the normal migration simulation conditions(37℃,salt concentration of 0.01 mol/L and pH 7 of saliva simulated solution),the migration coefficient of BPA and BPS was 1.3×10^(-17),6.6×10^(18)cm^(2)/s/s respectively.Based on enzyme immunoassay,the migration model of BPA and BPS in paper toys is constructed and successfully applied to predict the migration behavior of bisphenol compounds in paper toys.According to the migration simulation experiment of bisphenol in paper toys,the diffusion coefficients of bisphenol under different conditions are calculated and the migration model is fitted to predict the migration of BPA and BPS in blind samples.In comparison with the actual migration,the theoretical prediction value is basically consistent with the actual migration amount.The work is of great significance for risk assessment of bisphenol compounds in children’s paper toys.