基于IPTW法筛选高脂血症人群的差异肠道菌群

Screening Discrepant Gut Microbiota of Hyperlipidemia Based on IPTW

目的:研究高脂血症患者与非高脂血症人群肠道菌群的差异。方法:采用Illumina HiSeq二代测序技术分析肠道菌群组成和丰度,利用逆处理概率加权法(IPTW)校正混杂因素,通过QIIME2 ANCOM筛选高脂血症的差异菌群,运用R Tax4Fun包进行功能预测。结果:高脂血症组和非高脂血症组共有7个差异扩增序列变体(ASVs)(ASV_232、ASV_63、ASV_173、ASV_46、ASV_393、ASV_283、ASV_41);甘油三酯组和非甘油三酯组(TG组与非TG组)只有1个差异ASV_(ASV_422);高密度脂蛋白胆固醇组与非高密度脂蛋白胆固醇组(HDL-C组和非HDL-C组)共有18个差异ASVs(ASV_9、ASV_15、ASV_28、ASV_36、ASV_50、ASV_56、ASV_80、ASV_99、ASV_153、ASV_45、ASV_636、ASV_243、ASV_109、ASV_516、ASV_13、ASV_74、ASV_96、ASV_259)。功能预测结果显示:高脂血症组与非高脂血症组样本菌群功能组成高度相似,没有发现显著差异,大多数功能基因与能量代谢有关。结论:高脂血症患者与非高脂血症人群的差异肠道菌群为毛螺菌科、疣微菌科,双歧杆菌属、粪杆菌属、拟杆菌属、瘤胃菌属_UCG-013、Agathobacter、Subdoligranulum、Lachnoclostridium、Lachnospiraceae_UCG-010属,该结果说明碳水化合物代谢和氨基酸代谢所占比例较大。

Objective:To study the discrepancy of gut microbiota between patients with hyperlipidemia and people without hyperlipidemia.Methods:Illumina HiSeq second-generation sequencing technology was used to analyze the gut microbiota composition and abundance,and the inverse probability of treatment weighting(IPTW)method was used to correct confounding factors.The discrepant gut microbiota of hyperlipidemia was screened by QIIME2 ANCOM,and the R Tax4 Fun package was used for functional prediction.Results:There were 7 discrepant ASVs between the hyperlipidemia group and the non-hyperlipidemia group(ASV_232、ASV_63、ASV_173、ASV_46、ASV_393、ASV_283、ASV_41).There was only one discrepant ASV_ between the TG group and the non-TG group(ASV_422).There were 18 discrepant ASVs between the HDL-C group and the non-HDL-C group(ASV_9、ASV_15、ASV_28、ASV_36、ASV_50、ASV_56、ASV_80、ASV_99、ASV_153、ASV_45、ASV_636、ASV_243、ASV_109、ASV_516、ASV_13、ASV_74、ASV_96、ASV_259).Functional prediction results showed that the functional composition of the sample in the hyperlipidemia group and the non-hyperlipidemia group was highly similar,and no significant difference was found.Most functional genes were related to energy metabolism.Conclusion:The discrepancy of gut microbiota between patients with hyperlipidemia and people without hyperlipidemia is Lachnospiraceae、Ruminococcaceae at family level,Bifidobacterium,Faecalibacterium,Bacteroides,Ruminococcaceae_UCG-013,Agathobacter,Subdoligranulum,Lachnoclostridium,Lachnospiraceae_UCG-010 at genus level,functional prediction showed that carbohydrate metabolism and amino acid metabolism accounted for a large proportion.