白薇薄层鉴别的方法改进

Improvement of TLC Identification Method for Cynanchi atrati Radix Et Rhizoma

目的根据现有药典标准,改进白薇的薄层鉴别方法,更加有效的控制续断药材质量。方法用硅胶G板为薄层板,甲苯-二氯甲烷-乙酸乙酯-冰醋酸(15∶5∶7∶0.5)为展开剂。以白薇对照药材为对照品,对白薇药材进行薄层色谱鉴别条件的研究。结果薄层色谱斑点较多且清晰、分离度好、耐用性好,能有效鉴别白薇药材。结论改进方法与药典相比,改进方法专属性强,展开斑点多且清晰,可替代现有标准用于白薇药材的鉴别。

OBJECTIVE According to the existing pharmacopoeia standard,this article aimed to control the quality of Cynanchi atrati Radix Et Rhizoma more effectively by improving the TLC identification method of Cynanchi atrati Radix Et Rhizoma.METHODS Using silica gel G thin layer plate,and toluene dichloromethane ethyl acetate glacial acetic acid(15∶5∶7∶0.5)as developing agent,the TLC identification conditions of Cynanchi atrati Radix Et Rhizoma were studies with Cynanchi atrati Radix Et Rhizoma contrast as the reference standard.RESULTS The Cynanchi atrati Radix Et Rhizoma could be effectively identified with more and clear TLC spots,good resolution and durability.CONCLUSION Compared with the Pharmacopoeia,the improved method is more specific with more and clear TLC spots,which can replace the existing standards for the identification of Cynanchi atrati Radix Et Rhizoma.