PD-L1、MMP-3在老年食管鳞癌组织中的表达及其相关性

Expression and correlation of PD-L1 and MMP-3 in elderly esophageal squamous cell carcinoma

目的分析程序性死亡受体-配体1(PD-L1)、基质金属蛋白酶-3(MMP-3)在老年食管鳞癌组织中的表达及其相关性,为老年食管癌患者早期疾病诊断、治疗方案制定及预后评估提供有效依据。方法回顾性分析青海省交通医院2018年10月至2019年10月收治的经手术病理证实的83例老年食管鳞癌患者临床资料,将其纳入食管鳞癌组。另取46例患者的癌旁组织并将其纳入癌旁组。采取免疫组化法对比两组组织中PD-L1、MMP-3阳性表达率,分析食管鳞癌组织中PD-L1、MMP-3表达与临床病理关系,经双变量Pearson相关性检验分析二者与老年食管鳞癌临床病理特征的相关性。结果PD-L1、MMP-3在食管鳞癌组阳性表达率(55.42%、67.47%)高于癌旁组(23.91%、30.43%),差异有统计学意义(P<0.05);经Pearson相关性分析发现,PD-L1表达与食管鳞癌分化程度呈正相关(r=0.449,P<0.001),MMP-3表达与食管鳞癌浸润深度、TNM分期、淋巴结转移呈正相关(r=0.255、0.367、0.361,P=0.020、0.001、0.001)。结论PD-L1及MMP-3在老年食管鳞癌中呈高表达,而PD-L1表达可提示食管鳞癌分化程度,MMP-3表达可提示食管鳞癌不同浸润程度、TNM分期及淋巴结转移情况,PD-L1及MMP-3因子可作为临床中食管鳞癌治疗方案制定及预后评估标志物。

Objective To analyze the expression and correlation of programmed death receptor lig-and 1(PD-LI)and matrix metalloproteinase-3(MMP-3)in elderly esophageal squamous cell carcinoma,in order to provide an effective basis for early disease diagnosis,treatment plan formulation and prognosis e-valuation of elderly patients with esophageal cancer.Methods The clinical data of 83 elderly patients with esophageal squamous carcinoma who admitted in Qinghai Prov incial Traffic Hospital from October 2018 to October 2019 were retrospectively analyzed and ineluded in the esophageal squamous carcinoma group.An-other 46 patients with para-carcinoma tissue were included in the para-carcinoma group.The immunohisto-chemistry was used to compare the positive rate of PD-LI and MMP-3 expression in the two groups of tis-sues,and the relationship between PD-L1 and MMP-3 expression in esophageal squamous carcinoma tssues and clinical pathology was explored.Bivariate Pearson correlation test was used to analyze the correlation between the two and the clinical pathological features of elderly patients with esophageal squamous careino-ma.Results The positive expression rates of PD-L1 and MMP-3 in the esophageal squamous careinoma group(55.42%,67.47%)were higher than those in the para-carceinoma group(23.91%,30.43%)(P<0.05);Pearson correlation analysis found that PD-L1 expression was positively correlated with the dif-ferentiated degree of esophageal squamous carcinoma(r=0.449,P<0.001),and MMP-3 expression was positively correlated with the infiltration degree of esophageal squamous carcinoma,tumor node metastasis(TNM)stage,and lymph node metastasis(r=0.255,0.367,0.361,P=0.020,0.001,0.001).Conclusions PD-LI and MMP-3 are highly expressed in elderly esophageal squamous carcinoma.PD-LIexpression can indicate the degree of differentiation of esophageal squamous cearcinoma,and MMP-3 expres-sion can indicate different infiltration degrees of esophageal squamous carcinoma,TNM stage,and lymph node metastasis.PD-LI and MMP-3 can be used as clinical markers for the development of esophageal squamous carcinoma treatment plans and prognostic evaluation.