基于血浆蛋白组学的维生素D补充改善糖尿病的作用

Plasma proteomic analysis of diabetes improvement by vitamin D supplementation

目的基于血浆蛋白组学,分析维生素D补充对2型糖尿病的改善作用。方法5周龄SPF级雄性自发性肥胖型2型糖尿病大鼠20只,按体重随机分为糖尿病组和糖尿病维生素D干预组,对照组为Zucker瘦型大鼠,喂养至12周龄,比较各组大鼠空腹血糖,高分辨质谱系统iTRAQ定量分析比较各组大鼠血浆蛋白质,并进行KEGG信号通路分析。结果糖尿病维生素D干预组大鼠空腹血糖显著低于糖尿病组,糖尿病组表达差异的蛋白质在糖尿病维生素D干预组得到了较明显的改善,KEGG信号通路分析发现,糖尿病组差异蛋白主要分布于酶类、外泌体蛋白以及肽酶和抑制剂,并且糖尿病干预组的这3类蛋白的差异数量明显降低。结论维生素D补充可以改善糖尿病大鼠空腹血糖及血浆蛋白质的表达差异情况。

OBJECTIVE To analyze the effect of vitamin D supplementation on the improvement of diabetes mellitus based on plasma proteomics.METHODS Five-week-old SPF spontaneously obese rats with type 2 diabetes were randomly divided into a diabetic group and a diabetic vitamin D intervention group,and the control group was Zucker lean rats.The fasting blood glucose of the rats in each group was compared with that of the diabetic vitamin D group,and the plasma proteins of the rats in each group were compared by quantitative analysis of the high-resolution mass spectrometry system iTRAQ,and KEGG signaling pathway analysis was performed.RESULTS The fasting blood glucose of rats in the diabetic vitamin D intervention group was significantly lower than that of the diabetic group,and the proteins that were differentially expressed in the diabetic vitamin D intervention group were significantly improved.KEGG signaling pathway analysis revealed that the differential proteins in the diabetic group were mainly distributed among enzymes,exosomal proteins,and peptidases and inhibitors,and that the number of differences in these three classes of proteins was significantly reduced in the diabetic intervention group.CONCLUSION Vitamin D supplementation can improve the differential expression of fasting glucose and plasma proteins in the diabetic rats.