增加餐后体能活动对于初发2型糖尿病患者代谢基因表观遗传的影响

The Effect of Increasing Postprandial Physical Activity on the Epigenetics of Metabolic Genes in Patients with Newly Diagnosed Type 2 Diabetes

目的探究运动干预对于初发2型糖尿病患者代谢基因的表观遗传影响。方法选取2018年12月-2020年12月该院收治的40例初发2型糖尿病患者作为研究对象。两周运动干预后,比较增加体能活动前后的生化代谢指标及炎症、代谢基因的表观遗传变化。结果增加体能活动后患者血糖、血脂、血压改善均优于增加体能活动前,差异有统计学意义(P<0.05);腰围、臀围、体质量、BMI比较,差异无统计学意义(P>0.05);POMC、GLUT 4、miR-138、miR-15b、miR-376a、miR-130b 6个基因的甲基化程度均较前降低;TFAM、FOXA2、CCL2、ICAM1、LEP、DAT等6个基因的甲基化程度均较前升高,差异有统计学意义(P<0.05);NPY、TH基因的甲基化程度较前比较,差异无统计学意义(P>0.05)。增加体能活动前不同HbA1c分组之间的炎症及代谢基因的甲基化水平比较,差异无统计学意义(P>0.05)。结论增加餐后体能活动可能通过调控初发2型糖尿病患者代谢基因的甲基化程度参与维持患者内分泌及代谢功能的稳态平衡。

Objective To explore the epigenetic effects of increased postprandial physical activity on metabolic genes in patients with type 2 diabetes.Methods 40 cases of newly diagnosed type 2 diabetes from in the hospital December 2018 to December 2020 were selected as the research subjects.After 2 weeks of exercise intervention,compared the biochemical and metabolic indicators before and after increasing physical activity and the epigenetic changes of inflammation and metabolic genes.Results The improvement of blood glucose,blood lipid and blood pressure after increasing physical activity was better than that before increasing physical activity,and the difference was statistically significant(P<0.05).There was no significant difference in waist circumference,hip circumference,body mass and BMI(P>0.05).The methylation degree of POMC,GLUT 4,miR-138,miR-15b,miR-376a,miR-130b 6 genes were significantly lower than those befor.the methylation levels of TFAM,FOXA2,CCL2,ICAM1,LEP and DAT genes were significantly higher than those before,and the difference was statistically significant(P<0.05).There was no significant difference in the methylation degree of NPY and the genes(P>0.05).There was no significant difference in the methylation levels of inflammatory and metabolic genes between different HbA1c groups before increasing physical activity(P>0.05).Conclusion Increasing postprandial physical activity may be involved in maintaining the homeostasis of endocrine and metabolic functions by regulating the methylation of metabolic genes in patients with type 2 diabetes.