六例由副溶血性弧菌引起的食物中毒的调查分析

Investigation and Analysis of 6 Cases of Food Poisoning Caused by Vibrio Parahaemolyticus

目的:通过实验室的病原学检测和毒力基因确认,来寻找引起食物中毒的原因。方法:开展流行病学调查,并根据GB 4789.7-2013《食品安全国家标准食品微生物学检验》对6例疑似食物中毒的病例采集肛门拭子分别进行沙门氏菌、志贺氏菌、霍乱狐菌、蜡样芽胞杆菌、金黄色葡萄球菌、副溶血性弧菌分离培养鉴定和提取细菌组DNA进行实时荧光PCR反应。结果:6份采集的肛拭子中,沙门氏菌、志贺氏菌、霍乱狐菌、蜡样芽胞杆菌、金黄色葡萄球菌和致泻性大肠埃希氏菌的培养及荧光PCR结果均为阴性。而副溶血性弧菌培养,质谱鉴定和荧光PCR结果均为阳性,且均携带耐热直接溶血素(TDH)和不耐热溶血毒素(TLH)毒力基因。结论:结合实验室检测及流行病学调查,确认引起此次食物中毒的病原为副溶血性弧菌。

Objective:To find out the cause of food poisoning through laboratory etiology test and virulence gene confirmation.Methods:Epidemiological investigations were carried out,and according to GB 4789.7-2013 National Food Safety Standard Food Microbiology Inspection,anal swabs were collected from 6 suspected food poisoning cases for Salmonella,Shigella,Cholera fox,and cereus.Bacillus,Staphylococcus aureus,and Vibrio parahaemolyticus were isolated,cultured and identified,and bacterial group DNA was extracted for real-time fluorescent PCR reaction.Results:Among the 6 collected anal swabs,the culture and fluorescence PCR results of Salmonella,Shigella,Fox cholera,Bacillus cereus,Staphylococcus aureus and diarrheal Escherichia coli were all negative.The results of Vibrio parahaemolyticus culture,mass spectrometry identification and fluorescence PCR were all positive,and they all carried thermostable direct hemolysin(TDH) and thermolabile hemolysin(TLH) virulence genes.Conclusion:Combined with laboratory tests and epidemiological investigations,it is confirmed that the pathogen causing food poisoning was Vibrio parahaemolyticus.