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河南省境外输入新型冠状病毒肺炎病例的病毒基因组特征分析 被引量:1

Genomic characteristics of 2019 novel coronavirus from imported cases of coronavirus disease 2019 in Henan Province
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摘要 目的分析河南省境外输入2019新型冠状病毒(2019 novel coronavirus,2019-nCoV)毒株的基因组进化特征及变异情况。方法纳入2020年5月至12月河南省报告的16例境外输入新型冠状病毒肺炎确诊病例,采集患者咽拭子标本送至河南省疾病预防控制中心进行全基因组测序。以全球共享流感数据倡议组织平台上公布的SARS-CoV-2 Wuhan-Hu-1作为参考序列,采用MEGA X软件进行序列比对和分析,并采用最大似然法构建系统进化树。结果16例病例中,13例来自俄罗斯,2例来自缅甸,1例来自乌克兰。共获得16条基因组长度为29804~29882 bp的2019-nCoV基因组序列。共检测到145个核苷酸突变位点和80个氨基酸突变位点,所有序列均存在C241T、C3037T、C14408T、A23403G核苷酸位点突变,以及刺突蛋白区D614G氨基酸位点突变。BetaCov/HEN02/Human/2020、BetaCov/HEN04/Human/2020和BetaCov/HEN05/Human/2020毒株基因组的第29704位点出现碱基A插入,所有序列均未发现缺失变异。系统进化树分析显示,16株毒株与目前流行的需关注的变异株均无相关性。结论2020年5月至12月河南省境外输入病例的2019-nCoV毒株基因组突变呈现随机性和多样性,均不属于需关注的变异株。 Objective To analyze the evolutionary characteristics and variations of 2019 novel coronavirus(2019-nCoV)strains imported from abroad in Henan Province.Methods A total of 16 imported cases of coronavirus disease 2019(COVID-19)reported in Henan Province from May to December 2020 were enrolled.The throat swab specimens from the patients were collected and sent to the Henan Provincial Center for Disease Control and Prevention for whole genome sequencing.Taking SARS-CoV-2 Wuhan-Hu-1 published in Global Initiative on Sharing All Influenza Data(GISAID)as the reference sequence,the sequences were aligned and analyzed by MEGA X,and the phylogenetic tree was constructed by the maximum likelihood method.Results Among 16 cases,13 cases were imported from Russia,two cases were imported from Myanmar,and one case was imported from Ukraine.A total of 16 strains of 2019-nCoV genomes with the lengths of 29804 bp to 29882 bp were obtained.A total of 145 nucleotide mutations and 80 amino acid mutations were detected.Nucleotide variations of C241T,C3037T,C14408T,A23403G and the amino acid variation of D614G in spike protein were detected in all sequences.Meanwhile,insertion A at the site of 29704 was found in BetaCov/HEN02/Human/2020,BetaCov/HEN04/Human/2020 and BetaCov/HEN05/Human/2020.Deletion variation was not found.Phylogenetic analysis showed that there was no correlation between the 16 strains and currently epidemic variants of concern(VOC).Conclusion From May to December 2020,the detection of viral genome mutations in the imported cases of Henan Province shows randomness and diversity,while the strains are not VOC.
作者 毋碧聪 宋云 卢世栋 胡晓 马红霞 李东晓 李懿 王海霞 叶莹 黄学勇 郭万申 Wu Bicong;Song Yun;Lu Shidong;Hu Xiao;Ma Hongxia;Li Dongxiao;Li Yi;Wang Haixia;Ye Ying;Huang Xueyong;Guo Wanshen(Henan Key Laboratory of Infectious Disease Microbiology,Department of Prevention and Control of Infectious Disease,Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,China)
出处 《中华传染病杂志》 CAS CSCD 2021年第11期664-669,共6页 Chinese Journal of Infectious Diseases
基金 河南省省部共建重大专项(SBGJ202001003) 新型冠状病毒防控应急攻关首批项目(201100310800) 河南省中青年卫生健康科技创新领军人才培养项目(YXKC2020006) 河南省疾病预防控制中心科研基金资助项目(01030455)。
关键词 冠状病毒属 基因组 序列比对 2019新型冠状病毒 进化 关切变异株 Coronavirus Genome Sequence alignment 2019 novel coronavirus Evolution Variant of concern
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