GEO数据库分析环状RNA在急性髓细胞白血病中的差异表达

Analysis of the Differential Expression of circRNA in Acute Myeloid Leukemia by GEO Database

目的:采用生物信息学方法探究环状RNA(circRNA)在急性髓细胞白血病(AML)中的差异表达。方法:从美国国家生物信息中心(NCBI)的基因表达综合库(GEO)下载AML的基因芯片数据,利用R语言软件对正常对照及AML样本进行差异分析,然后利用miranda软件和miRTarBase软件对差异表达circRNA、miRNA和mRNA之间的相互作用进行预测。通过Cytoscape插件构建circRNA-miRNA-mRNA关系对相关的ceRNA网络。结果:通过差异分析,最终获得了203个差异表达circRNA,包括161个circRNA下调,42个circRNA上调;通过软件预测构建了circRNA/miRNA/mRNA互相作用网络;通过基因异常表达分析发现,hsa;irc;001080、hsa;irc;004511、hsa;irc;054211、hsa;irc;001944可能正向调控AML的基因表达。结论:在AML中异常表达circRNA可能成为AML治疗的新靶点。

Objective: To investigate the difference expression of circular RNA( circRNA) in acute myeloid leukemia( AML) by using bioinformatics method. Methods: The microarray chip data of AML was searched and downloaded from the Gene Expression Omnibus( GEO) of the National Center for Bioinformatics( NCBI). The differences between AML samples and control samples were analyzed by R software. The interaction between deregulated circRNA,miRNA and mRNA were predicted by miranda software and miRTarBase software. The circRNA-miRNAmRNA regulatory network was constructed by using the cytoHubba plugin based on the Cytoscape software. Results: A total of 203 differential expression of circRNAs were finally collected,including down-regulated 161 circRNAs and upregulated42 circRNAs. CircRNA/miRNA/mRNA interaction network was constructed through software prediction. hsa;irc;001080,hsa;circ;0004511,hsa;circ;0054211,hsa;circ;0001944 may be positively regulated the gene expression in AML. Conclusion: Abnormal expression of circRNA in AML may become a new target for AML treatment.