急性髓系白血病脓毒症患者外周血lncRNA-MALAT1表达情况及其临床意义

Expression of LncRNA-MALAT1 in Peripheral Blood of Patients with Acute Myeloid Leukemia Sepsis and Its Clinical Significance

目的:分析急性髓系白血病(AML)脓毒症患者外周血lncRNA-MALAT1的表达并探讨其临床意义。方法:选择2018年3月至2019年3月进入龙泉驿区第一人民医院肿瘤血液科治疗的确诊为AML的患者95例,其中包括43例脓毒症感染者和52例未感染者,取外周血作为研究样本,以50例健康人血液样本作为对照。采用RT-qPCR技术对健康组、未感染组、脓毒症组样本中的lncRNA-MALAT1进行定量,分析lncRNA-MALAT1表达水平与AML脓毒症患者临床特征和预后的关系。结果:与健康组和未感染组相比,脓毒症组患者外周血中lncRNA-MALAT1的表达显著上调(P<0.05),健康组和未感染组之间lncRNA-MALAT1的表达水平比较无显著差异(P>0.05);在脓毒症组患者中,lncRNA-MALAT1的表达与患者NCCN风险分级、白细胞数、血红蛋白等临床特征相关;lncRNA-MALAT1高表达组的总生存率显著低于低表达组(χ^(2)=23.157,P=0.002);COX回归分析表明,lncRNA-MALAT1可以作为AML脓毒症患者独立的预后因素。结论:lncRNA-MALAT1在AML脓毒症患者中的表达上调,与患者临床特征和生存率密切相关,是AML脓毒症独立的预后因素,有望成为AML脓毒症患者新的诊断标志物和治疗靶点。

Objective: To analyze the expression of lncRNA-MALAT1 in peripheral blood of patients with acute myeloid leukemia(AML) sepsis and explore its clinical significance. Methods: From March 2018 to March 2019, 95 confirmed AML patients including 43 sepsis infected cases and 52 uninfected cases were selected for treatment in the Department of Oncology and Hematology, The First People′s Hospital of Longquanyi District. Their peripheral blood samples were taken as study samples, and the blood samples from 50 healthy people were used as control. RT-qPCR was used to detect lncRNA-MALAT1 expression level in samples from healthy group, uninfected group, and sepsis group. The correlation between lncRNA-MALAT1 expression level and clinical characteristics and prognosis of AML patients with sepsis were analyzed. Results: The expression level of lncRNA-MALAT1 in the sepsis group was significantly up-regulated compared with the healthy group and uninfected group(P<0.05), while there was no significant difference between the healthy group and uninfected group(P>0.05). In AML patients with sepsis, the expression of lncRNA-MALAT1 was associated with clinical characteristics such as NCCN risk classification, white blood cell count, hemoglobin and so on.The overall survival rate of high lncRNA-MALAT1 expression group was significantly lower than that of low expression group(χ^(2)=23.157, P=0.002). COX regression analysis showed that lncRNA-MALAT1 could be an independent prognostic factor for AML sepsis patients. Conclusion: The up-regulated expression of lncRNA-MALAT1 is closely related to the clinical characteristics and survival rate, and is an independent prognostic factor for AML sepsis patients. LncRNAMALAT1 is expected to become a new diagnostic marker and therapeutic target for AML sepsis.