CSN6在食管鳞状细胞癌中的表达及临床意义

The expression and clinical significance of CSN6 in esophageal squamous cell carcinoma

目的探讨食管鳞状细胞癌中组成型光形态发生因子9信号复合体亚单位6(CSN6)的表达及临床意义。方法选择2019年1月—2020年12月本院80例食管鳞状细胞癌患者、68例Barrett食管患者、120名健康体检者作为研究对象,收集所有研究对象的血清,采用酶联免疫吸附试验(ELISA)检测血清中CSN6蛋白的表达量,采用电化学发光法检测癌胚抗原(CEA)、鳞状细胞癌相关抗原(SCCA)的表达量。绘制ROC曲线比较上述指标的诊断价值。采用免疫组织化学实验检测2013年9月—2016年1月本院病理科存档的食管鳞状细胞癌手术标本共180例,检测癌及癌旁石蜡组织标本中CSN6的表达,分析CSN6与临床病理特征及预后的关系。结果食管鳞状细胞癌患者血清中CSN6的表达量高于Barrett食管患者和健康体检者,差异具有统计学意义(P<0.05)。ROC曲线表明:CSN6、CEA和SCCA的诊断效能低于三者联合检测,差异具有统计学意义(P<0.05)。食管鳞状细胞癌组织中CSN6的阳性率与癌旁正常组织比较,差异具有统计学意义,CSN6的阳性表达与肿瘤大小、病理分期、浸润深度、淋巴结转移有关(P<0.05)。CSN6阳性表达患者的5年生存率与阴性表达比较,差异具有统计学意义(P<0.05)。多因素分析表明CSN6阳性表达是判断食管鳞状细胞癌不良预后的独立危险因素(P<0.05)。结论CSN6可能作为食管鳞状细胞癌早期诊断、预测不良预后的分子标志物。

Objective To investigate the expression and clinical significance of constitutive photomorphogenesis 9 signalosome subunit 6(CSN6)in esophageal squamous cell carcinoma.Methods From January 2019 to December 2020,80 patients with esophageal squamous cell carcinoma,68 patients with Barrett's esophagus,and 120 healthy people those underwent health examination were enrolled as study objects,sera were collected from all the objects,the enzyme-linked immunosorbent assay(ELISA)was used to detect the expression level of CSN6 in the serum,while the electrochemiluminescence method was used to detect cancer embryo antigen(CEA)and squmaous cell carcinoma antigen(SCCA).The receiver operating characteristic(ROC)curve was drew to compare the diagnostic value of the above mentioned indicators.The expression of CSN6 in 180 esophageal squamous cell carcinoma and para-carcinoma paraffin tissue specimens collected in our hospital from September 2013 to January 2016 were detected by the immunohistochemistry.We analyzed the relationship between CSN6 and clinicopathological characteristics and prognosis.Results The expression of CSN6 in serum of patients with esophageal squamous cell carcinoma was higher than that of patients with Barrett's esophagus carcinomaand healthy objects,the differences were statistically significant(P<0.05).The ROC curve showed that the diagnostic efficacy of CSN6,CEA or SCCA alone in the diagnosis of esophageal squamous cell carcinomawas lower than the combined detection of them,the differences were statisticallysignificant(P<0.05).The positive rate of CSN6 in esophageal squamous cell carcinoma tissue was higher than that in normal tissues adjacent to the cancer,the differences were statistically significant(P<0.05).The positive expression of CSN6 was related to tumor size,pathological stage,depth of invasion,and lymph node metastasis(P<0.05).The 5-year survival rate of patients with positive expression of CSN6 was lower than that of negative expression patients(P<0.05).Multivariate analysis showed that the positive expression of CSN6 is an independent risk factor for the poor prognosis of esophageal squamous cell carcinoma(P<0.05).Conclusions CSN6 may be used as a molecular marker for early diagnosis and poor prognosis of esophageal squamous cell carcinoma.