不同产地凤丹籽乙醇提取物中多酚含量及其抗氧化、降糖活性

Polyphenols content,antioxidant and hypoglycemic activities of Fengdan seed ethanol extract from different producing areas

以4个产地(陕西宝鸡、山东菏泽、安徽亳州、安徽铜陵)凤丹籽为研究对象,采用福林酚法测定凤丹籽提取物(90%乙醇提取)中多酚含量,以DPPH、ABTS+自由基清除实验评价其抗氧化活性,通过对α-葡萄糖苷酶的抑制作用评价其降糖活性,并运用液相色谱-加热电喷雾电离串联质谱法(LC-MS/MS-HESI)对活性最佳的凤丹籽提取物中的多酚类物质进行分析。结果表明:安徽铜陵凤丹籽提取物多酚含量最高,为40.69 mg/g;4个产地凤丹籽提取物均具有一定抗氧化活性和降糖活性,且抗氧化活性和降糖活性从强到弱依次为安徽铜陵>安徽亳州>山东菏泽>陕西宝鸡;从安徽铜陵凤丹籽提取物中鉴定出24种多酚类化合物。安徽铜陵凤丹籽提取物的多酚含量及抗氧化、降糖活性优于其他3个产地,拥有良好的应用前景。

Fengdan seeds from four producing areas(Baoji(Shaanxi),Heze(Shandong),Bozhou(Anhui),Tongling(Anhui))were used as the research objects,the polyphenols content of Fengdan seed ethanol extract was determined by Folin-Ciocalteu method.The antioxidant activity of the extract was evaluated by DPPH and ABTS+radical scavenging experiments,and the hypoglycemic activity was evaluated by studying the inhibitory effect onα-glycosidase enzymes.Finally,the polyphenols compounds in the Fengdan seed extract with the best activity were analyzed by LC-MS/MS-HESI.The results showed that the content of polyphenols in Fengdan seed extract from Tongling,Anhui was the highest(40.69 mg/g).The Fengdan seed extracts from four producing areas had certain antioxidant and hypoglycemic activities,and the activity of Fengdan seed extract from Tongling,Anhui was the best,followed by Bozhou(Anhui),Heze(Shandong)and Baoji(Shaanxi).A total of 24 polyphenols compounds were identified from Fengdan seed extract from Tongling,Anhui.The polyphenols content,antioxidant and hypoglycemic activities of Fengdan seed extract from Tongling,Anhui were better than those of the other three producing areas,and had a good application prospect.