水中诺如病毒的两种富集方法的效果比较

Comparison on effects of two enrichment methods for norovirus in water

目的利用MCE-PEG(混合硝酸纤维素膜富集和聚乙二醇)沉淀法和超滤法富集水中诺如病毒,评估超滤法对水中诺如病毒的富集效果。方法向1 L纯净水中加入一定量的诺如病毒进行稀释后,分别利用MCE-PEG沉淀法和超滤法对诺如病毒进行富集,最后用实时荧光定量PCR技术检测并计算诺如病毒的回收率。结果MCE-PEG法对稀释度超过一万倍的水样中的诺如病毒几乎无富集作用,超滤法富集对一万倍稀释度的水样的病毒平均回收率为34.72%,对十万倍稀释度的水样病毒平均回收率为6.21%。结论超滤法比MCE-PEG沉淀法富集病毒的效果更好。

Objective Mixed nitrocellulose membrane enrichment and polyethylene glycol(MCE-PEG)precipitation and ultrafiltration methods were used to enrich norovirus in water,aiming to evaluate the enrichment effect of ultrafiltration on the norovirus in water.Methods A certain amount of norovirus was added to the purified water(1 L)to dilute,and MCE-PEG precipitation and ultrafiltration methods were respectively used to enrich the norovirus.Finally,the recovery rate of norovirus was detected and calculated by using real-time PCR technology.Results MCE-PEG methods had almost no enrichment effect on norovirus in water samples with the dilutability greater than 10000 folds.The recovery rate of ultrafiltration in water samples with the dilutability of ten-thousand-time was 34.72%and that in the water sample with the dilutability of one-hundred-thousand folds was 6.21%.Conclusion For norovirus enrichment,the ultrafiltration method is more effective than MCE-PEG precipitation method.