华中枸骨根化学成分研究

Chemical constituents from roots of Ilex centrochinensis

目的研究华中枸骨Ilex centrochinensis根的化学成分。方法运用硅胶、Sephadex LH-20,ODS柱色谱及半制备HPLC等方法进行分离纯化,并通过NMR等波谱技术和理化性质鉴定结构。结果从华中枸骨根中分离得到13个化合物,分别鉴定为11-羰基-ɑ-香树醇-3β-棕榈酸酯(1)、3β-乙酰氧基-28-羟基-12-烯-乌苏烷(2)、儿茶酚(3)、4-乙基儿茶酚(4)、2,4,6-三甲氧基苯酚(5)、3-(4-羟基-3,5-二甲氧基苯基)-1,2-丙二醇(6)、3-(4-羟基-3-甲氧基苯基)-1,2-丙二醇(7)、3,4-二甲氧基苯酚(8)、3,4-二羟基苯丙酸甲酯(9)、3β,19α,23-三羟基-20α(H)-乌苏-12-烯-28-酸3β-O-α-L-吡喃阿拉伯糖(10)、(+)-1-羟基松脂醇-1-O-β-D-葡萄糖苷(11)、3β-O-[α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖]-坡模酸(12)、rotundioic acid(13)。结论所有化合物均为首次从华中枸骨中分离得到,其中4~9为首次从冬青属植物中分离得到。

Objective To investigate the chemical constituents from the roots of Ilex centrochinensis.Methods The chemical constituents were isolated and purified by silica gel,Sephadex LH-20,ODS and semi-prepared high performance liquid chromatography and their structures were elucidated on the basis of NMR technique and physicochemical property.Results Thirteen compounds were obtained from the roots of I.centrochinensis,and named as 11-carbonyl-ɑ-amyrin-3β-palmitate(1),3β-acetoxy-28-hydroxy-12-ene-urs(2),catechol(3),4-ethyl catechol(4),2,4,6-trimethoxyphenol(5),3-(4-hydroxy-3,5-dimethoxyphenyl)propane-1,2-diol(6),3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol(7),3,4-dimethoxyphenol(8),3,4-dihydroxybenzenepropionic acid methyl ester(9),3β,19α,23-trihydroxy-20α(H)-urs-12-en-28-oic acid 3β-O-α-L-arabinopyranoside(10),(+)-1-hydroxypinoresinol-1-O-β-D-glucoside(11),3β-O-[α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl]-pomolic acid(12),rotundioic acid(13).Conclusion All compounds were isolated from I.centrochinensis for the first time,and compounds 4-9 were isolated from Ilex for the first time.