柔肝滋阴颗粒对焦虑抑郁性高血压大鼠的作用及机制研究

Effect and mechanism of Rougan Ziyin Granules on anxiety-depression hypertensive rats

目的研究柔肝滋阴颗粒对焦虑抑郁性高血压大鼠的作用及机制。方法采用慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)刺激制备焦虑抑郁性高血压大鼠,给予柔肝滋阴颗粒进行干预,比较各组大鼠体质量、糖水消耗率和旷场实验行为学变化;测量各组大鼠收缩压和舒张压;采用ELISA法测定大鼠血清及海马组织中白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平,采用免疫比浊法测定大鼠血清超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)水平。结果与对照组比较,模型组大鼠体质量、糖水消耗率、中央格停留时间、穿越格数和站立次数均明显减少(P<0.01);收缩压和舒张压明显升高(P<0.05、0.01);血清及海马组织IL-6、TNF-α水平明显升高(P<0.01);血清hs-CRP水平明显升高(P<0.01)。与模型组比较,柔肝滋阴颗粒组大鼠体质量、糖水消耗率、中央格停留时间、穿越格数、站立次数均明显增加(P<0.05、0.01);收缩压和舒张压明显降低(P<0.05);血清及海马组织IL-6、TNF-α水平明显降低(P<0.05、0.01);血清hs-CRP水平明显降低(P<0.01)。结论柔肝滋阴颗粒能够明显改善焦虑抑郁性高血压大鼠焦虑抑郁症状并降低血压,其机制可能与下调炎症因子IL-6、TNF-α、hs-CRP水平有关。

ObjectiveTo study the effect and mechanism of Rougan Ziyin Granules(柔肝滋阴颗粒)on anxiety-depressive hypertensive rats.Methods Chronic unpredictable mild stress(CUMS)stimulation was used to prepare anxiety-depressive hypertensive rats,Rougan Ziyin Granules was given for intervention.Body weight,sugar water consumption rate and open field experimental behavior changes ofrats ineach group were compared;Systolic and diastolic blood pressure of rats in each group weremeasured;ELISA method was used to detect interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels in serum and hippocampus tissueof rats;Immunoturbidimetric method was used to determine high-sensitivity C-reactive protein(hs-CRP)levelinserumof rats.Results Compared with control group,body weight,sugar water consumption rate,central grid residence time,number of crossing grids and standing times of rats inmodel group were significantly reduced(P<0.01);Systolic blood pressure and diastolic blood pressure were significantly increased(P<0.05,0.01);IL-6 and TNF-αlevels inserum and hippocampus tissue were significantly increased(P<0.01);hs-CRP level in serum wassignificantly increased(P<0.01).Compared with model group,body weight,sugar water consumption rate,central grid residence time,number of grids crossing and standing times of rats in Rougan Ziyin Granules group were increased(P<0.05,0.01);Systolic blood pressure and diastolic blood pressure were significantly reduced(P<0.05);IL-6 and TNF-αlevels in serum and hippocampus were significantly reduced(P<0.05,0.01);hs-CRP level in serum was reduced(P<0.01).Conclusion Rougan Ziyin Granules can significantly improve anxiety and depression symptoms and lower blood pressure in rats with anxiety-depressive hypertension,of whichmechanism may be related to down-regulation of inflammatory factors IL-6,TNF-αand hs-CRP.