一种直接破坏蟾蜍(蛙)脑和脊髓的新方法

A new method for directly destroying the brain and spinal cord of toads(frogs)

破坏蟾蜍(蛙)脑和脊髓方法是机能实验学课程中常用的基本实验操作技能之一,其传统法是通过枕骨大孔先后破坏脑和脊髓组织达到处死蟾蜍(蛙)目的,此种方法很大程度上凭感觉和经验才能一次成功,对于初学者来说,往往一次成功率很低。本文介绍的直接法可一次性直接将脑和脊髓完全破坏,简单易行,成功率高,适于教学中推广应用。

The destruction method of toad(frog)in brain and spinal cord is one of the basic experiment skills in the functional laboratory course.The traditional method is to damage the brain and spinal cord tissue respectively to kill toad(frog)through the foramen magnum.The successful operation of the traditional method mainly depends on handler’s feeling and experience in most cases.For beginner students,its successful rate of damaging brain and spinal cord tissue of toad(frog)once is often very low.While the direct method can destroy the brain and spinal cord of toads(frogs)at one time and has simple and feasible advantages and the higher success rate.The direct method destroying the brain and spinal cord of toads(frogs)is suitable for popularization and application in the teaching.