摘要
目的观察芪白平肺胶囊对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)痰瘀阻肺证模型大鼠肺部炎症和肺功能的影响。方法将80只雄性SD大鼠随机分为正常组、模型组、脾氨肽组、芪白平肺胶囊组。采用强迫游泳、被动吸烟和低氧环境复合方法复制COPD模型。观察大鼠一般状态;检测支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中细胞总数和炎症细胞比例;光学显微镜下观察肺组织形态变化;ELISA法检测大鼠血清白细胞介素(interleukin,IL)-1β、IL-6、IL-8及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;检测各组大鼠第0.3秒用力呼气容积(forced expiratory volume in 0.3 seconds,FEV0.3)、用力肺活量(forced vital capacity,FVC)、FEV0.3占FVC比值(FEV0.3/FVC)。结果与正常组比较,模型组大鼠一般情况较差,肺功能明显降低,肺组织可见大量炎症细胞浸润,肺泡间隔明显增厚,支气管壁可见明显黏液腺增生,管腔变窄;BALF中细胞总数、单核-巨噬细胞、中性粒细胞、淋巴细胞比例均明显升高(P<0.05);血清IL-1β、IL-6、IL-8、TNF-α水平明显升高(P<0.05)。与模型组比较,芪白平肺胶囊组及脾氨肽组大鼠肺组织病理变化明显改善;BALF中细胞总数和单核-巨噬细胞、中性粒细胞、淋巴细胞比例及血清IL-1β、IL-6、IL-8、TNF-α水平明显降低(P<0.05);FEV0.3、FVC、FEV0.3/FVC均明显升高(P<0.05)。其中芪白平肺胶囊组上述指标的改善程度明显大于脾氨肽组。结论芪白平肺胶囊可明显改善COPD痰瘀阻肺证大鼠肺部炎症,改善大鼠肺功能,其机制可能与下调血清相关炎症指标及肺部炎症细胞浸润有关。
Objective To investigate the effect of Qibai Pingfei Capsule on pulmonary inflammation and pulmonary function in rats with chronic obstructive pulmonary disease(COPD)with syndrome of phlegm and blood stasis obstructing the lung.Methods A total of 80 male Sprague-Dawley rats were randomly divided into normal group,model group,spleen aminopeptide group,and Qibai Pingfei Capsule group.A rat model of COPD was established by forced swimming,passive smoking,and hypoxic environment.General status of the rats was observed;the total number of cells and the proportion of inflammatory cells in bronchoalveolar lavage fluid(BALF)were measured;lung histomorphological changes were observed under a light microscope;ELISA was used to measure the serum levels of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8),and tumor necrosis factor-α(TNF-α);related pulmonary function parameters were measured,including forced expiratory volume in 0.3 seconds(FEV0.3),forced vital capacity(FVC),and FEV0.3/FVC ratio.Results Compared with the normal group,the model group had poor general status,a significant reduction in pulmonary function,marked inflammatory cell infiltration in lung tissue,significantly thickened alveolar septa,obvious mucous gland hyperplasia of the bronchial wall,and narrowing of the bronchial lumen,as well as significant increases in the total number of cells and the proportion of monocyte-macrophages,neutrophils,and lymphocytes in BALF and the serum levels of IL-1β,IL-6,IL-8,and TNF-α(P<0.05).Compared with the model group,the Qibai Pingfei Capsule group and the spleen aminopeptide group had significant improvements in the pathological changes of lung tissue,significant reductions in the total number of cells and the proportion of monocyte-macrophages,neutrophils,and lymphocytes in BALF and the serum levels of IL-1β,IL-6,IL-8,and TNF-α(P<0.05),and significant increases in FEV0.3,FVC,and FEV0.3/FVC ratio(P<0.05).The Qibai Pingfei Capsule group had significantly greater improvements in the above indices than the spleen aminopeptide group.Conclusion Qibai Pingfei Capsule can significantly improve pulmonary inflammation and pulmonary function in COPD rats with syndrome of phlegm and blood stasis obstructing the lung,possibly by downregulating serum inflammatory markers and inflammatory cell infiltration in lungs.
作者
朱张求
石孟瑶
方莉
朱洁
李泽庚
童佳兵
ZHU Zhang-qiu;SHI Meng-yao;FANG Li;ZHU Jie;LI Ze-geng;TONG Jia-bing(Anhui University of Chinese Medicine, Anhui Hefei 230012, China;The First Affiliated Hospital of Anhui University of Chinese Medicine, Anhui Hefei 230031, China;Institute of Respiratory Disease Prevention and Treatment of Traditional Chinese Medicine, Anhui Academy of Chinese Medicine, Anhui Hefei 230031, China)
出处
《安徽中医药大学学报》
CAS
2021年第6期52-57,共6页
Journal of Anhui University of Chinese Medicine
基金
国家自然科学基金项目(81974569)
安徽省自然科学基金青年项目(1908085QH340)。
关键词
慢性阻塞性肺疾病
痰瘀阻肺证
芪白平肺胶囊
白细胞介素
肿瘤坏死因子
肺功能
Chronic obstructive pulmonary disease
Syndrome of phlegm and blood stasis obstructing the lung
Qibai Pingfei Capsule
Interleukin
Tumor necrosis factor
Pulmonary function