黑果腺肋花楸花青素的分离纯化

Separation, Purification of Anthocyanins from Aronia prunifolia ’Viking’

通过比较4种大孔树脂对黑果腺肋花楸花青素的吸附-解吸性能而筛选出最佳纯化树脂,研究花青素在该树脂中的静态吸附动力学模型、颗粒扩散模型、吸附-解吸条件的影响因素、动态吸附泄露曲线和洗脱曲线,并采用液相色谱对纯化的花色苷进行结构解析。结果表明, HPD-100大孔树脂对黑果腺肋花楸花青素有良好的纯化效果,其吸附花青素过程符合准二级动力学模型,并以薄膜扩散为主要吸附方式。该树脂对花青素的静态吸附-解吸条件为:花青素平衡吸附量2.73 mg/g树脂、花青素酸化甲醇提取液pH 3.0、吸附时间4.5 h;解吸液乙醇体积分数60%、pH 3.0、解吸时间2.5 h。动态吸附-解吸条件为:花青素提取液上样量4 BV、上样流速700μL/min、洗脱量5 BV、解吸流速500μL/min。黑果腺肋花楸花青素经纯化后分离出4种花色苷单体,其中已鉴定出的3种分别为矢车菊素-3-半乳糖苷(49.04%)、矢车菊素-3-阿拉伯糖苷(41.17%)和矢车菊素-3-木糖苷(6.45%)。

The optimal macroporous resin for anthocyanin derived from Aronia prunifolia ‘Viking’ purification was selected by means of adsorption and desorption properties of 4 macroporous resins comparison. The static adsorption dynamics model, particle diffusion model and factors influence of adsorption-desorption conditions of anthocyanins in the resin were studied. The leak curve and elution curve of dynamic adsorption were explored in succession. The structure of anthocyanin constituents were identified through HPLC-DAD. The results showed that HPD-100 macroporous resin was the optimal resin used for anthocyanin purification, and its adsorption process of anthocyanin was in line with the quasi-second-order kinetic model, while membrane diffusion was the main adsorption modality. The static anthocyanin adsorption-desorption condition of the resin was as follows: the equilibrium adsorption capacity of anthocyanin 2.73 mg/g, the acidified methonal solvent pH 3.0,and adsorption duration 4.5 h;the volume fraction of desorption ethanol solution 60%, its pH 3.0, and the desorption duration 2.5 h. The dynamic adsorption-desorption condition was as follows: the sample loading quantity of anthocyanin extract 4 BV,the sample loading velocity 700 μL/min, the elution quantity 5 BV, and the desorption velocity 500 μL/min. Four anthocyanin monomers were isolated from Aronia prunifolia ‘Viking’ anthocyanins after purification, and three of them were identified as cyanidin 3-galactoside(49.04%), cyanidin-3 arabinoside(41.17%) and cyanidin-3 xyloside(6.45%).