口腔微生物稳态与口腔鳞癌患者顺铂敏感性的相关性分析

Correlation between oral microbial homeostasis and cisplatin sensitivity in patients with oral squamous cell carcinoma

目的:探讨口腔鳞癌(oral squamous cell carcinoma,OSCC)患者口腔内微生物与顺铂敏感性之间的相关性。方法:采集29例OSCC患者化疗前唾液样本,根据RECIST 1.1评价标准将患者分为顺铂耐药组(无效)和顺铂敏感组(有效),采用16S r RNA基因测序,分析OSCC患者口腔微生物物种组成、群落多样性及丰度、样本间差异。采用SPSS 19.0软件包对数据进行统计学处理,LEfSe分析2组样本间物种差异。结果:与顺铂化疗敏感组相比,顺铂耐药组唾液样本中微生物多样性和丰度显著增加。在属水平上,顺铂耐药组患者口腔内奇异菌属、红蝽菌、微单胞菌、尤里优杆菌以及梭杆菌与顺铂敏感组相比存在显著差异。结论:顺铂耐药组与敏感组OSCC患者口腔内微生物结构和组成存在显著差异,提示微生物可能成为OSCC患者顺铂化疗前筛选的生物标志物。

PURPOSE:To explore the correlation between oral microbial homeostasis and cisplatin sensitivity in patients with oral squamous cell carcinoma(OSCC).METHODS:Saliva samples were collected from 29 OSCC patients before chemotherapy,according to RECIST 1.1 evaluation criteria,the recruited patients were sorted into two groups:cisplatin re-sistant group(ineffective)and cisplatin sensitive group(effective).16 S r RNA gene sequencing was used to analyze the species composition,community diversity,abundance of oral microorganisms and comparison between samples.The data were analyzed with SPSS 19.0 software package,and LEfSe was used to analyze the species difference between the two groups.RESULTS:Compared with cisplatin chemotherapy-sensitive group,the microbial diversity in saliva samples of the cisplatin resistant group increased significantly.At genus level,Atopobium,Coriobacteriaceae,Parvimonas,Eubacterium yurii and Fusobacteriumin in the chemotherapy-resistant group were significantly increased compared with the sensitive group.CONCLUSIONS:There is significant difference in the structure and composition of oral microorganisms be-tween cisplatin resistant group and cisplatin sensitive group,suggesting that microbes may become biomarkers before chemotherapy for patients with OSCC.