摘要
细胞谱系示踪技术是研究干细胞特性的重要工具,基于Cre/Loxp重组酶系统的谱系示踪技术是示踪方法的一大突破,组成性重组酶系统实现了报告基因的组织特异性,诱导性重组酶系统在此基础上实现了报告基因表达时间的可控性。研究成骨改建机制涉及成骨细胞系命运,对骨改建研究、组织工程学应用是有重要意义。本文综述了基于Cre/Loxp重组酶系统的谱系示踪技术在研究成骨系细胞分化、来源、转归及其在发育和修复中的功能等方面的应用,并探讨其适用范围、优势和不足。
Cell lineage tracing technology is an important tool to study the characteristics of stem cells,among which,the one based on Cre/Loxp recombinase system is a great breakthrough.General recombinase system realizes the tissue specificity of reporter gene,based on which,inducible recombinase system makes controlling the expression time of reporter gene possible.The study of the mechanism of osteogenic remodeling is related to the fate of osteoblast lines,which is of great significance to the study of bone remodeling and the application of tissue engineering.This paper reviewed the application of lineage tracing technique based on Cre/Loxp recombinase system in the study of the origin,differentiation,lineage and hierarchy of osteoblast lines and their function in development and repair,and discussed its scope of application,advantages and disadvantages.
作者
徐宁蔚
王晶
龚佳静
张瑾
金安婷
江凌勇
XU Ning-wei;WANG Jing;GONG Jia-jing;ZHANG Jin;JIN An-ting;JIANG Ling-yong(College of Stomatology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011 China;Department of Oral and Maxillofacial Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011 China)
出处
《中国口腔颌面外科杂志》
CAS
2021年第6期562-567,共6页
China Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金(81570950,81870740
81800949)
上海市优秀学术/技术带头人计划(20XD1422300)
上海市“医苑新星”青年医学人才培养资助计划—杰出青年医学人才项目(HWJRS2019-72)
上海交通大学医学院高水平创新团队激励计划-协同创新团队激励项目
上海交通大学医学院第十四期大学生创新训练计划(1420Y507)
上海交通大学医学院附属第九人民医院“交叉”研究基金项目·重点项目(JYJC201902)。
