妊娠期牙周感染IL-1β基因rs1143634和rs16944位点多态性

Polymorphisms of rs1143634 and rs16944 loci of IL-1β gene in patients with periodontal infection during pregnancy

目的探讨妊娠期牙周感染患者白细胞介素-1β(IL-1β)基因rs1143634和rs16944位点多态性。方法选择武汉市第四医院口腔科2019年1月-2020年12月存在牙周感染孕妇150例为研究组,选择同期医院进行牙周检查为健康孕妇100名为对照组,研究组根据牙周炎严重程度分为轻度牙周炎76例、中/重度牙周炎74例。限制性片段长度多态性聚合酶链反应IL-1β基因rs1143634和rs16944位点多态性,分析两位点基因多态性对孕妇牙周感染易感性及牙周炎严重程度的影响。结果研究组与对照组rs1143634位点基因型、等位基因分布差异无统计学意义;研究组rs16944位点CC基因型、C等位基因频率高于对照组,差异具有统计学意义(P<0.05);中/重度牙周炎孕妇与轻度牙周炎孕妇rs1143634位点基因型、等位基因分布差异无统计学意义;中/重度牙周炎孕妇rs16944位点CC基因型、C等位基因频率高于轻度牙周炎孕妇,TT基因型频率低于轻度牙周炎孕妇,差异具有统计学意义(P<0.05)。结论 IL-1β基因rs16944位点CC基因型显著增加本地区女性孕期牙周感染易感性,且与疾病严重程度相关。

OBJECTIVE To explore the polymorphisms of rs1143634 and rs16944 locus of interleukin-1β( IL-1β) gene in patients with periodontal infection during pregnancy. METHODS A total of 150 pregnant women with periodontal infection who were treated in stomatology department of West Hospital of Wuhan Fourth Hospital from Jan 2019 to Dec 2020 were assigned as the study group, meanwhile, 100 healthy pregnant women who received periodontal examination were chosen as the control group. The patients of the study group were divided into the mild periodontitis group with 76 cases and the moderate/severe periodontitis group with 74 cases. The polymorphisms at rs1143634 and rs16944 loci of IL-1β gene were detected by restriction fragment length polymorphism polymerase chain reaction(PCR), and the effect of the gene polymorphisms at the two loci on the susceptibility to periodontal infection and severity of periodontitis was observed. RESULTS There was no significant difference in the distribution of genotypes and alleles at rs1143634 locus between the study group and the control group. The frequencies of CC genotype and C allele at rs16944 locus were significantly higher in the study group than in the control group(P<0.05). There was no significant difference in the distribution of genotypes and alleles at rs1143634 locus between the moderate/severe periodontitis patients and the mild periodontitis patients;the frequencies of CC genotypes and C allele at rs16944 locus were higher in the moderate/severe periodontitis pregnant women than in the mild periodontitis pregnant women, the frequency of TT genotypes of the moderate/severe periodontitis pregnant women was significantly lower than that of the mild periodontitis pregnant women(P<0.05). CONCLUSION The CC genotype at rs16944 locus of IL-1β gene may significantly increase the pregnant women’s susceptibility to periodontal infection in the area and is associated with the severity of disease.