不同工艺提取的大黄外用对肺炎支原体感染小鼠肺部炎症的影响

Effects of Different Extraction Processed Rhei Radix et Rhizoma on Pulmonary Inflammation in Mice Infected with Mycoplasma Pneumoniae

目的探讨不同工艺提取的大黄外用对肺炎支原体(Mycoplasma pneumoniae,MP)感染小鼠JAK2/STAT3信号通路的影响,探究其改善小鼠肺部炎症的效应靶点。方法将40只BALB/c小鼠随机分为正常组、模型组、生粉组、醇提组,每组10只。除正常组小鼠外,其余3组采用滴鼻法进行MP感染。造模后,将各组对应药物贴于小鼠背俞穴区30 min·d^(−1),连续敷药7 d。给药后第7天取材,光镜下观察小鼠肺组织、皮肤病理切片,计算肺组织病理评分、肺指数,采用ELISA法检测血清及肺组织中IL-6含量,Western blotting及qPCR检测STAT3、JAK2蛋白和mRNA的表达。结果MP感染后小鼠肺泡间隔增厚,细支气管壁增厚,炎性细胞浸润,小鼠肺指数、肺组织炎症病理评分、血清及肺组织中IL-6含量、肺组织中STAT3和JAK2的蛋白表达及mRNA含量均升高(P<0.05)。治疗后小鼠肺指数、肺组织炎症病理评分、血清及肺组织中IL-6含量均降低(P<0.05),且醇提组作用效果较生粉组明显,但血清中含量差异不显著。与模型组比较,醇提组、生粉组STAT3、JAK2蛋白及mRNA含量下降(P<0.05)。结论大黄醇提物外用可有效改善MP感染小鼠肺部炎症,下调IL-6、JAK2及STAT3的表达,为其治疗MP肺炎提供了参考。

OBJECTIVE To explore the effects of external application of Rhei Radix et Rhizoma extracted by different processes on JAK2/STAT3 signaling pathway in mice infected with Mycoplasma pneumonia(MP),and to investigate the target of improving pulmonary inflammation in mice.METHODS Forty BALB/c mice were randomly divided into normal group,model group,raw powder group and ethanol extract group,with 10 mice in each group.Except the normal mice,the other 3 groups were infected with MP by nasal dropping.After the model was established,the corresponding drugs of each group were applied to the Back-Shu acupoint area of mice for 30 min·d^(−1) for 7 consecutive days.On the 7th day after administration,pathological sections of lung and skin of mice were observed under light microscopy.Lung histopathological scores and lung index were calculated.IL-6 content in serum and lung tissue was detected by ELISA.STAT3,JAK2 protein and mRNA expression were detected by Western blotting and qPCR.RESULTS After MP infection,the alveolar septum,bronchiolar wall,inflammatory cell infiltration,lung index,inflammatory pathological score of lung tissue,IL-6 content in serum and lung tissue,the protein expression and mRNA content of STAT3 and JAK2 in lung tissue of mice were all increased(P<0.05).After treatment,lung index,pathological score of pulmonary inflammation,IL-6 content in serum and lung tissue of mice were decreased(P<0.05),and the effect of ethanol extraction group was more obvious than that of raw powder group,but there was no significant difference in serum content.Compared with model group,the contents of STAT3,JAK2 protein and mRNA in ethanol extract group and raw powder group decreased(P<0.05).CONCLUSION External application of Rhei Radix et Rhizoma ethanol extract can effectively improve the pulmonary inflammation in mice infected with MP and down-regulate the expression of IL-6,JAK2 and STAT3,which provides reference for its treatment of MP pneumonia.