扎龙湿地10个不同居群芦苇的ITS序列分析

ITS Sequence Analysis of 10 Different Populations of Phragmites australis in Zhalong Wetland

对扎龙湿地10个不同居群普通芦苇和铁秆芦苇的ITS序列进行了分析。结果表明,10种不同居群芦苇碱基含量差异不大,G+C含量在69.2%~71.1%之间。DNA密度高,遗传信息稳定。铁秆芦苇居群存在287~290个变异位点,普通芦苇居群只存在3个变异位点,序列较为保守。10个不同居群芦苇平均遗传距离为0.302 6,两型芦苇遗传距离为0.905 7~0.909 0,两型芦苇遗传信息有差别且遗传距离相对较远。构建的MP系统树和NJ系统树结果一致,即居群4与8同属铁秆芦苇,其余居群为普通芦苇。芦苇通过适应环境发生基因的变异而演化出了新的生态型:铁秆芦苇。

In this study, the ITS sequences of common Phragmites australis and Tiegan P. australis from 10 different populations in Zhalong Wetland were analyzed. The results showed that there was little difference in the content of G+C among the 10 populations, and the content of G+C ranged from 69.2% to 71.1%, and these populations had high DNA density and stable genetic information. There were 287-290 mutation loci in the population of Tiegan R. australis, while there were only 3 mutation loci in the population of common R. australis. The average genetic distance between the 10 populations was 0.302 6, and the genetic distance between the two types was 0.905 7-0.909 0. The genetic information of the two types was different and the genetic distance was relatively long. The results of MP system tree and NJ system tree were consistent, that was, populations 4 and 8 belonged to Tiegan R. australis, and the other populations were common R. australis. In conclusion, a new ecotype, Tiegan R. australis, was developed by adaptation gene mutation.