活化T细胞核因子5对人胃癌MGC803细胞迁移、侵袭及细胞周期的影响

Effects of nuclear factor of activated T cells 5 on migration,invasion and cell cycle of human gastric cancer MGC803 cells

背景:近年研究发现,活化T细胞核因子5(nuclear factor 5 of activated T cells,NFAT5)高表达与多种肿瘤的发生进展关系密切。前期研究发现,NFAT5能够促进人胃癌MGC803细胞增殖并抑制其凋亡。目的:探讨NFAT5对人胃癌MGC803细胞迁移力、侵袭力及细胞周期的影响。方法:实验分为siRNA阴性对照组与NFAT5-siRNA组。有效转染人胃癌MGC803细胞沉默NFAT5基因表达后,采用划痕实验、Transwell实验和流式细胞术分别检测各组细胞迁移力、侵袭力及细胞周期分布的改变。结果与结论:(1)siRNA阴性对照组划痕的愈合能力明显高于NFAT5-siRNA组;(2)NFAT5-siRNA组穿过小室的细胞数目明显少于siRNA阴性对照组[(134.63±25.62)个vs.(195.00±60.41)个,P<0.05];(3)与siRNA阴性对照组相比,NFAT5-siRNA组细胞中G;期细胞比例显著升高[(60.03±0.55)%vs.(62.46±0.73)%,P<0.05],而S期细胞比例显著降低[(28.24±1.16)%vs.(25.44±1.15)%,P<0.05];(4)提示沉默人胃癌MGC803细胞中NFAT5基因表达能够有效减弱细胞的迁移力和侵袭力,改变细胞周期分布,进而减少细胞增殖,NFAT5有望成为胃癌诊治的新靶点。

BACKGROUND:Recent studies have shown that high expression of nuclear factor 5 of activated T cells(NFAT5)is closely related to the occurrence and progression of a variety of tumors.Previous studies have found that NFAT5 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC803 cells.OBJECTIVE:To investigate the effects of NFAT5 on migration,invasion and cell cycle of human gastric cancer MGC803 cells.METHODS:There were two groups:NC-si RNA and NFAT5-si RNA.The si RNAs were transfected to silence NFAT5 gene expression in MGC803 cells.Cell scratch assay,Transwell assay and flow cytometry were performed to test migration ability,invasion ability,and cell cycle distribution,respectively.RESULTS AND CONCLUSION:The wound healing capacity of NC-si RNA group was significantly higher than that in the NFAT5-si RNA group.The number of cells passed through the ventricle in the NFAT5-si RNA group was obviously less than that in the NC-si RNA group[(134.63±25.62)vs.(195.00±60.41),P<0.05].Compared with the NC-si RNA group,the proportion of G;-phase cells was significantly increased[(60.03±0.55)%vs.(62.46±0.73)%,P<0.05]and the proportion of S-phase cells was significantly decreased[(28.24±1.16)%vs.(25.44±1.15)%,P<0.05].Therefore,NFAT5 silencing in human gastric cancer MGC803 cells can inhibit cell migration and invasion abilities in vitro and change the cell cycle distribution,thereby reducing cell proliferation ability.NFAT5 is expected to be a new diagnostic and therapeutic target for gastric cancer.