复发性阿弗他溃疡患者外周血淋巴细胞亚群指标、免疫球蛋白及补体水平的变化及意义

Changes of lymphocyte subsets,immunoglobulin and complement levels in peripheral blood of patients with recurrent aphthous ulcer

目的探讨免疫因素与复发性阿弗他溃疡(RAU)发病的关系。方法选取2019年10月至2020年9月在宁夏医科大学总医院就诊的RAU患者123例作为RAU组。另外,选取同期体检的健康者120例作为对照组。采用流式细胞术检测淋巴细胞亚群指标CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)百分比,CD4^(+)/CD8^(+),CD3-CD16^(+)CD56^(+)、CD3-CD19^(+)百分比;采用免疫散射比浊法检测体液免疫相关指标免疫球蛋白IgA、IgG、IgM及补体C3、C4水平。采用SPSS20.0统计软件对上述指标的检测结果进行组间比较。结果RAU组与对照组外周血淋巴细胞CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)百分比,CD4^(+)/CD8^(+),CD3-CD16^(+)CD56^(+)、CD3-CD19^(+)百分比比较,差异均无统计学意义(P>0.05);RAU组免疫球蛋白IgA、IgG和补体C3、C4水平高于对照组,差异均有统计学意义(P<0.05)。结论RAU患者的细胞免疫与体液免疫功能均存在异常,免疫因素可能参与了RAU的发生和发展过程。

Objective To investigate the relationship between immune factors and the onset of recurrent aphthous ulcer(RAU).Methods A total of 123 RAU patients who were treated in the General Hospital of Ningxia Medical University from October 2019 to September 2020 were enrolled in the study as the RAU group.In addition to that,120 healthy people who underwent physical examinations during the same period were enrolled as the control group.Flow cytometry was used to detect lymphocyte subsets indicators CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+)percentage,CD4^(+)/CD8^(+),CD3-CD16^(+)CD56^(+),CD3-CD19^(+)percentage;immunoscattering turbidimetric method was used to detect humoral immunity related indicators immunoglobulins IgA,IgG,IgM and complements C3,C4 levels.The SPSS20.0 statistical software was used to compare the detection results of the above indicators between the two groups.Results Compared between RAU group and control group,there was no significant difference in the CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+)percentage,CD4^(+)/CD8^(+),CD3-CD16^(+)CD56^(+),CD3-CD19^(+)percentage(P>0.05);the levels of immunoglobulin IgA,IgG and complements C3 and C4 in the RAU group were higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion Both the cellular and humoral immune functions of RAU patients are abnormal,and immune factors may be involved in the occurrence and development of RAU.