一次性使用卫生用品中绿脓杆菌的鉴定分析与质量控制

Identification, Analysis and Quality Control of Pseudomonas aeruginosa in Disposable Sanitary Products

为满足新冠肺炎疫情下一次性使用卫生用品卫生状况的检测需求,参加一次性使用卫生用品中绿脓杆菌的检测能力验证,提升绿脓杆菌的检测能力。依据GB 15979-2002《一次性使用卫生用品卫生标准》中附录B4,采用手工生化试验、全自动微生物生化鉴定系统和PCR-荧光探针法对能力验证样品分离菌株和4株绿脓杆菌分离菌株进行鉴定。结果显示,样品20DP-2112未检出绿脓杆菌,样品20DP-1630检出绿脓杆菌,分离菌株A、B、C、D鉴定为绿脓杆菌;20DP-1630分离菌株和分离菌株A、B在3种方法中都呈现出典型的阳性结果,分离菌株C、D在手工生化鉴定中未呈现出典型的阳性特征,但全自动微生物生化鉴定系统和荧光PCR仪都显示为阳性结果,综合分析判定为绿脓杆菌。因此,绿脓杆菌的检验不能仅以手工生化试验为判定依据,还需结合多种方法才能实现对检测结果的质量控制。

In order to meet the demand for testing the sanitary conditions of disposable sanitary products during the COVID-19 pandemic and improve the detection capacity of Pseudomonas aeruginosa, we participated in "Verification of detection ability of Pseudomonas aeruginosa in disposable sanitary products". According to Hygienic standard for disposable sanitary products(GB 15979-2002 Appendix B4), the strains isolated from the proficiency testing samples and four isolates of Pseudomonas aeruginosa were identified by manual biochemical tests, automatic microbial biochemical identification system and PCR-fluorescence probe method. The results showed that no Pseudomonas aeruginosa was detected in sample 20 DP-2112, Pseudomonas aeruginosa strains were isolated from sample 20 DP-1630 and four strains A/B/C/D. Strains isolated from 20 DP-1630 and two strains A/B showed excellent positive results by all the methods. The other two strains C/D showed weak positive characteristics by manual biochemical identification, but both automatic microbial biochemical identification system and fluorescence PCR instrument showed high positive coincidence rate, and comprehensive analysis can also be determined as Pseudomonas aeruginosa. Therefore, the detection of Pseudomonas aeruginosa should not only rely on the result of manual biochemical tests, but also need to combine with a variety of methods to achieve the quality control of the test results.