趋化因子CCL21基因rs2812377单核苷酸多态位点与急性心肌梗死发病相关性分析

The association between the rs2812377 single nucleotide polymorphic site of chemokine CCL21 and acute myocardial infarction

目的探讨趋化因子CCL21基因rs2812377单核苷酸多态位点与急性心肌梗死(AMI)发病的相关性。方法选取自2012年4月至2018年10月北部战区总医院心血管内科收治的253例AMI患者纳入B组。另选取211例同期住院且冠状动脉造影显示冠状动脉的狭窄程度≤20%者纳入A组。采用酶联免疫吸附实验检测两组血浆中的CCL21表达水平。采用Agena Mass ARRAY技术对CCL21基因rs2812377单核苷酸多态位点进行分析,比较两组CCL21基因rs2812377单核苷酸多态位点基因型及等位基因的分布频率。结果两组研究对象趋化因子CCL21基因rs2812377单核苷酸多态位点3种基因型(TT型、GT型、GG型)及等位基因(T、G)分布频率比较,差异均有统计学意义(P<0.05)。CCL21基因rs2812377单核苷酸多态位点G等位基因是AMI发病的独立危险因素(比值比=1.36,95%可信区间1.05~1.77,P<0.05)。B组血浆中CCL21水平明显高于A组,差异有统计学意义(P<0.05)。B组GG型、GT型、TT型血浆CCL21表达水平均高于A组,差异有统计学意义(P<0.05)。两组GG型、GT型血浆CCL21表达水平均高于TT型,差异有统计学意义(P<0.05)。结论CCL21基因rs2812377单核苷酸多态位点与AMI发生相关,CCL21基因rs2812377可以作为AMI的生物标志物。

Objective To investigate the rs2812377 single nucleotide polymorphic site of chemokine CCL21 gene with the incidence of acute myocardial infarction(AMI).Methods A total of 253 AMI patients admitted to the department of cardiology in General Hospital of Northern Theater Command from April 2012 to October 2018 were selected and included in Group B.Another 211 hospitalized patients with coronary artery stenosis≤20%by coronary angiography were included in group A.Plasma CCL21 expression levels in both groups were detected by enzyme-linked immunosorbent assay.The rs2812377 single nucleotide polymorphism of CCL21 gene was analyzed by Agena Mass ARRAY technique,and the difference of genotype and allele distribution frequency of rs2812377 single nucleotide polymorphism of CCL21 gene was compared between the two groups.Results There were statistically significant differences in the distribution frequencies of 3 genotypes(TT type,GT type,GG type)and alleles(T,G)of rs2812377 single nucleotide polymorphic loci of CCL21 gene between the two groups(P<0.05).The single nucleotide polymorphic site G allele of CCL21 gene rs2812377 was an independent risk factor for AMI(odds ratio=1.36,95%CI:1.05-1.77,P<0.05).The level of CCL21 in group B was significantly higher than that in group A,the difference was statistically significant(P<0.05).The level of CCL21 in GG,GT and TT types in group B were higher than those in group A,with statistical significance(P<0.05).The level of CCL21 in GG type and GT type in group A and B were higher than those in TT type,and the differences were statistically significant(P<0.05).Conclusion rs2812377 single nucleotide polymorphism of CCL21 gene was associated with the occurrence of AMI,and RS2812377 of CCL21 gene could be used as a biomarker of AMI.