HPLC同时测定斑秃丸中11种活性组分

Simultaneous Determination of 11 Kinds of Active Components in Bantu Pills by HPLC

目的建立高效液相色谱(HPLC)同时测定斑秃丸中芍药苷、毛蕊花糖苷、二苯乙烯苷、阿魏酸、丹酚酸B、五味子醇甲、大黄素、异欧前胡素、五味子甲素、丹参酮IIA和五味子乙素含量的方法。方法采用HPLC,Venusil MP C18色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,流速为1.0 ml/min;进样量为10μl;检测波长为220 nm;柱温为35℃。结果芍药苷在51.98~1559.46 ng(r=1.0000),毛蕊花糖苷在21.84~655.20 ng(r=1.0000),二苯乙烯苷在103.60~3108.00 ng(r=0.9999),阿魏酸在6.79~203.67 ng(r=1.0000),丹酚酸B在79.80~2393.91 ng(r=1.0000),五味子醇甲在20.94~628.20 ng(r=1.0000),大黄素在7.13~213.81 ng(r=1.0000),异欧前胡素在18.16~544.80 ng(r=1.0000),五味子甲素在6.71~201.15 ng(r=1.0000),丹参酮IIA在3.25~97.44 ng(r=0.9999),五味子乙素在7.96~238.80 ng(r=1.0000)进样量范围内与峰面积呈良好线性关系;平均加样回收率分别为97.64%,102.61%,96.95%,98.23%,95.64%,99.53%,97.90%,99.02%,103.55%,102.31%,100.24%,RSD均<5%(n=6)。结论本方法专属性强,灵敏度高,可有效控制斑秃丸的质量。

Objective To develop an HPLC method for the determination of paeoniflorin, verbascoside, stilbene glucoside,ferulic acid, salvianolic acid B, schisandrin, emodin, isoimperatorin, deoxyschizandrin, tanshinone IIA and schisandrin B in Bantu Pills at the same time. Methods The chromatographic separation was achieved on a Venusil MP C18 column(4.6 mm×250 mm, 5 μm), using acetonitrile-0.1 % phosphoric acid solution at the flow rate of 1.0 ml/min as mobile phase for gradient elution. The injection amount was 10 μl, the detection wavelength was set at 220 nm, and column temperature was 35 ℃. Results Paeoniflorin, verbascoside, stilbene glucoside, ferulic acid, salvianolic acid B, schisandrin, emodin,isoimperatorin, deoxyschizandrin, tanshinone IIA and schisandrin B showed good linear relationships between injection amount and peak area at the ranges of 51.98-1559.46 ng(r=1.0000), 21.84-655.20 ng(r=1.0000), 103.60-3108.00 ng(r=0.9999), 6.79-203.67 ng(r=1.0000), 79.80-2393.91 ng(r=1.0000), 20.94-628.20 ng(r=1.0000), 7.13-213.81 ng(r=1.0000), 18.16-544.80 ng(r=1.0000), 6.71-201.15 ng(r=1.0000), 3.25-97.44 ng(r=0.9999) and 7.96-238.80 ng(r=1.0000). The average recoveries were 97.64 %, 102.61 %, 96.95 %, 98.23 %, 95.64 %, 99.53 %, 97.90 %, 99.02 %,103.55 %, 102.31 % and 100.24 %, and RSDs were<5 %(n=6). Conclusion The established analytical method is of strong specificity and high sensitivity, and can be used efficiently in the quality control of Bantu Pills.