超高效液相色谱法同时测定正柴胡饮颗粒中8个成分含量

Simultaneous Determination of Eight Constituents of Zhengchaihuyin Granules by UPLC

目的建立超高效液相色谱(UPLC)同时测定正柴胡饮颗粒中芍药苷、甘草苷、橙皮苷、升麻素苷、5-O-甲基维斯阿米醇苷、甘草酸铵、柴胡皂苷a和柴胡皂苷d 8个成分含量的方法。方法色谱柱:Thermo Scientific Acclaim;RSLC 120 C;(2.1 mm×100 mm,2.2μm),流动相:乙腈-0.1%磷酸溶液,梯度洗脱;流速:0.2 ml/min;检测波长:250 nm和210 nm;柱温:30℃;进样量:2.0μl。结果芍药苷、甘草苷、橙皮苷、升麻素苷、5-O-甲基维斯阿米醇苷、甘草酸铵、柴胡皂苷a和柴胡皂苷d线性范围分别为42.83~713.8μg/ml(r=0.9995),3.182~53.03μg/ml(r=0.9997),51.84~864.0μg/ml(r=0.9994),1.700~28.33μg/ml(r=0.9993),0.9098~15.16μg/ml(r=0.9995),12.55~209.1μg/ml(r=0.9996),2.168~36.13μg/ml(r=0.9994),0.2826~4.710μg/ml(r=0.9997);平均加样回收率(n=6)分别为98.30%,98.75%,98.49%,98.71%,98.47%,98.75%,98.57%,98.42%(RSD<2.0%);精密度、重复性、稳定性(36 h)试验的RSD<2.0%(n=6)。结论所建立的多成分测定方法快捷、准确、重复性好,可用于正柴胡饮颗粒的质量控制。

Objective To develop an UPLC method for the simultaneous determination of 8 components in Zhengchaihuyin Granules including paeoniflorin, liquiritin, hesperidin, prim-O-glucosylcimifugin,5-O-methylvisammioside, ammonium glycyrrhizinate, saikosaponin a and saikosaponin d. Methods Thermo Scientific Acclaim;RSLC 120 C;(2.1 mm×100 mm, 2.2 μm) chromatographic column was adopted, using acetonitrile-0.1 %phosphoric acid solution at a flow rate of 0.2 ml/min as mobile phase of gradient elution. The detection wavelength was 250 nm and 210 nm, the column temperature was 30 ℃, and the sample size was 2.0 μl. Results The linear ranges of paeoniflorin, liquiritin, hesperidin, prim-O-glucosylcimifugin, 5-O-methylvisammioside, ammonium glycyrrhizinate, saikosaponin a and saikosaponin d were 42.83-713.8 μg/ml(r=0.9995), 3.182-53.03 μg/ml(r=0.9997),51.84-864.0 μg/ml(r=0.9994), 1.700-28.33 μg/ml(r=0.9993), 0.9098-15.16 μg/ml(r=0.9995), 12.55-209.1 μg/ml(r=0.9996), 2.168-36.13 μg/ml(r=0.9994) and 0.2826-4.710 μg/ml(r=0.9997), respectively;the average recoveries(n=6) were 98.30 %, 98.75 %, 98.49 %, 98.71 %, 98.47 %, 98.75 %, 98.57 % and 98.42 %, with RSDs all below 2.0 %.RSDs of precision, reproducibility and stability tests(36 h) were lower than 2.0 %(n=6). Conclusion The established multi-component determination method is fast, accurate and reproducible. It can be used for the quality control of Zhengchaihuyin Granules.