CLTCL1基因rs1061325位点多态性与中国汉族精神分裂症的关联分析

Association study of rs1061325 polymorphism of CLTCL1 gene with schizophrenia in Chinese Han population

目的探讨中国汉族人群网格蛋白重链1(CLTCL1)基因rs1061325多态性与精神分裂症的相关性。方法选取2007—2008年在上海交通大学医学院附属精神卫生中心门诊或住院的662例精神分裂症患者作为病例组,选取同期的414名健康志愿者作为对照组。采用TaqMan探针基因分型技术对两组的CLTCL1基因rs1061325位点进行分型,采用阳性与阴性症状量表(PANSS)对病例组进行精神症状严重程度评定,并分析PANSS评分与不同基因型的相关性。结果病例组与对照组的rs1061325等位基因和基因型频率分布比较,差异无统计学意义(χ^(2)=0.25、0.28,P=0.62、0.87);在共显性、显性、隐性、超显性、加性遗传模式下基因型分布差异也无统计学意义(P>0.05),rs1061325基因型多态性对精神分裂症患者的发病年龄[(24.15±6.71)岁]、病程[(31.23±9.49)年]和BMI[(26.46±5.28)kg/m^(2)]也均无明显影响(F=1.15、0.33、0.75,P=0.32、0.72、0.48),rs1061325基因型多态性与PANSS阳性症状分、阴性症状分、一般精神病理分及PANSS总分无相关性(F=1.26、0.37、0.34、0.49,P=0.29、0.69、0.72、0.62)。结论在中国汉族人群中,CLTCL1基因rs1061325位点与精神分裂症无关联。

Objective To explore the relationship between the rs1061325 polymorphism of clathrin heavy chain like 1(CLTCL1)gene and schizophrenia in Chinese Han population.Methods A total of 662 schizophrenia patients in the inpatient department and outpatient department of Shanghai Mental Health Center of Shanghai Jiao Tong University School of Medicine from 2007 to 2008 were selected as the case group.At the same time,a total of 414 healthy volunteers were recruited as the control group.The rs1061325 polymorphism of the CLTCL1 gene was genotyped by TaqMan genotyping assay.the severity of psychiatric symptoms in the case group were assessed by the Positive and Negative Symptom Scale(PANSS),and the correlation between PANSS score and different genotypes was analyzed.Results There was no significant difference in the frequency distribution of rs1061325 alleles and genotype between the case group and the control group(χ^(2)=0.25,P=0.62;χ^(2)=0.28,P=0.87).There was no significant difference in genotype distribution under codominant,dominant,recessive,over-dominant and log-additive models(P>0.05).The rs1061325 genotype polymorphism in patients with schizophrenia had no significant effect on the age of onset[(24.15±6.71)years],the course of the disease[(31.23±9.49)years]and BMI[(26.46±5.28)kg/m2](F=1.15,P=0.32;F=0.33,P=0.72;F=0.75,P=0.48).There was no correlation between rs1061325 genotype polymorphism and PANSS positive symptom score,negative symptom score,general psychopathology score and PANSS total score(F=1.26,P=0.29;F=0.37,P=0.69;F=0.34,P=0.72;F=0.49,P=0.62).Conclusions There was no association between rs1061325 polymorphism of CLTCL1 gene and schizophrenia in Chinese Han population.