小儿健脾膏对胃动力障碍大鼠胃肠组织中胃动素mRNA和蛋白表达的影响

Effect of Xiao’er Jianpi Plaster on Motilin mRNA and Protein Expression in Stomach and Intestine of Rats with Gastric Motility Disorder

目的研究比较小儿健脾膏优化方和原方对胃动力障碍大鼠的影响,探讨其可能的分子生物学机制。方法以胃排空率和小肠推进率为检测指标,分别测定小儿健脾膏原方和优化方对胃肠功能障碍大鼠胃肠功能的影响,并检测大鼠血清中胃动素(motilin,MTL)含量、胃液总酸度、胃蛋白酶活性,通过Western blotting实验测定大鼠胃肠组织中MTL mRNA和蛋白的相对表达量。结果小儿健脾膏原方和优化方对胃肠功能障碍大鼠的胃排空和肠推进均有促进作用,二者疗效相当。小儿健脾膏原方和优化方组大鼠血清中MTL含量、胃液总酸度、胃蛋白酶活性较模型组显著升高,胃窦和十二指肠中MTLm RNA和MTL蛋白表达较模型组明显上升,优化方促胃肠MTL蛋白表达作用强于原方。结论小儿健脾膏原方和优化方均有明显的促胃肠动力的作用,二者强度相当,其机制可能与促进MTL和胃蛋白酶分泌,提升胃窦和十二指肠MTL mRNA和蛋白表达有关。

OBJECTIVE To study and compare the effects of Xiao’er Jianpi plaster optimized prescription and original prescription,explore its possible molecular biological mechanism.METHODS The gastric emptying rate and small intestinal propulsion rate were used to detect the effects of the original and optimized prescriptions of Xiao’er Jianpi plaster on gastrointestinal function of rats with gastrointestinal dysfunction,and the content of motilin(MTL)in serum,total acidity of gastric juice,and stomach protease activity,the relative expression of MTL mRNA and protein in rat gastrointestinal tissues was determined by Western blotting experiment.RESULTS Both the original prescription and the optimized prescription of Xiao’er Jianpi plaster had promoting effects on gastric emptying and intestinal propulsion in rats with gastrointestinal dysfunction,and both had the same effect.The content of MTL in serum,total acidity of gastric juice,and pepsin activity of rats in the original and optimized formula of Xiao’er Jianpi plaster groups were significantly higher than those of the model group,and the expressions of MTL mRNA and MTL protein in the antrum and duodenum were significantly higher than those of the model group.The optimized formula promoted gastrointestinal MTL protein expression stronger than the original formula.CONCLUSION Both the original formula and the optimized formula of Xiao’er Jianpi plaster have obvious effects of promoting gastrointestinal motility,and their strengths are equivalent.The mechanism may be related to the promotion of MTL,pepsin secretion and the expression of MTL mRNA and protein in the antrum and duodenum.