长链非编码RNA LINC00662在神经母细胞瘤中的表达及生物学作用

Expression and biological role of long non-coding RNA LINC00662 in neuroblastoma

目的:探索LINC00662在神经母细胞瘤中的表达情况,并探究其对神经母细胞瘤细胞增殖、周期分布及侵袭转移能力等生物学行为的调控作用。方法:采用RT-qPCR方法检测40对神经母细胞瘤组织以及外周正常组织中LINC00662的表达,同时分析与患儿临床病理特征之间的关系;采用脂质体转染技术下调神经母细胞瘤细胞中LINC00662的表达,继而用荧光定量PCR、CCK-8、流式细胞术以及Transwell等实验,分析神经母细胞细胞的增殖、周期以及侵袭转移等生物学特性。结果:神经母细胞瘤患儿的肿瘤病灶中LINC00662的表达水平较其配对癌旁正常组织明显上调,且其高表达状态往往预示着患儿肿瘤分化程度低、肿瘤分期晚以及淋巴结侵犯。敲低LINC00662的表达后能诱导更多神经母细胞瘤细胞阻滞在G2/M期,进而抑制神经母细胞瘤细胞增殖以及侵袭转移能力。结论:LINC00662是神经母细胞瘤的潜在治疗靶点。

Objective:To investigate the expression of LINC00662 in neuroblastoma,and its regulatory effect on the biological behaviors,including proliferation,cell cycling,invasion and migration,of neuroblastoma.Methods:RT-qPCR was used to detect the expression of LINC00662 in 40 pairs of neuroblastoma tissues and peripheral normal tissues.The relationship between LINC00662 expression and the clinicopathological features in children was also analyzed.Liposome transfection was used to down-regulate the expression of LINC00662 in neuroblastoma cells.Then,fluorescence quantitative PCR,CCK-8 assay,flow cytometry and Transwell assay were used to analyze the biological characteristics of neuroblast cell in proliferation,cell cycling,invasion and migration.Results:The expression of LINC00662 in the tumor lesions of neuroblastoma was significantly up-regulated compared with the normal adjacent tissues,and the high expression frequently seemed to indicate low tumor differentiation,advanced tumor staging,and lymph node invasion.Knockdown of LINC00662 expression can induce more neuroblastoma cells arrested in G2/M phase,thereby inhibiting the proliferation,invasion and migration of neuroblastoma cells.Conclusion:LINC00662 is a potential therapeutic target for neuroblastoma.