针灸足三里对高尿酸血症模型小鼠血清尿酸、IL-10、TNF-α的影响

Effect of Acupuncture and Moxibustion at Zusanli(ST36)on Serum Uric Acid,IL-10 and TNF-αfor Hyperuricemia Mice

目的:观察针刺及艾灸"足三里"对高尿酸血症模型小鼠尿酸及炎性因子影响,初步探讨针灸治疗高尿酸血症的疗效及机制。方法:选取56只雄性昆明小鼠,随机分为空白组和造模组,采用氧嗪酸钾联合次黄嘌呤灌胃复制高尿酸血症模型,造模成功后随机分为模型组、针刺组、艾灸组,每组14只。选择"足三里"穴进行针刺或艾灸治疗,治疗2周后观测小鼠空腹体重、粪便形态,检测小鼠血清尿酸、IL-10、TNF-α含量。结果:试验期间组间空腹体重无显著差异,造模后小鼠出现糊状粪便,治疗结束后艾灸组粪便形态明显改善。模型组与空白组比较,SUA水平显著升高(P<0.01),IL-10水平显著下降(P<0.05);针刺组、艾灸组SUA含量均显著下降,与模型组比较有统计学差异(P<0.05),与空白组比较无统计学差异(P>0.05)。艾灸组IL-10含量升高,与模型组比较有统计学差异(P<0.01),与空白组比较无统计学差异(P>0.05)。针刺组IL-10含量显著低于其余三组(P<0.01)。TNF-α各组间无显著差异(P>0.05)。结论:针刺和艾灸可以降低高尿酸血症模型小鼠血清尿酸,对高尿酸血症有治疗作用;艾灸可以明显促进抗炎因子IL-10的表达,其降尿酸机制可能与改善肠道内环境有关。针刺可下调IL-10的表达,其降尿酸作用机制还需要进一步研究。

Objective:Observing the effect of acupuncture and moxibustion at Zusanli(ST36)on uric acid and inflammatory factors for hyperuricemia mice,and preliminarily exploring the efficacy and mechanism of acupuncture and moxibustion in the treatment of hyperuricemia.Methods:Fifty-six male Kunming mice were selected and randomly divided into a blank group and a modeling group.The hyperuricemia model was induced by intragastric administration of Potassium Oxonate and Hypoxanthine.After the modeling succeed,the modeling group was randomly divided into model,acupuncture and moxibustion group(n=14 in each group).Acupuncture or moxibustion was applied to ST36 for 2 weeks.Observed the fasting body weight and fecal characteristics of mice,and the level of uric acid,IL-10 and TNF-αin the mice serum.Results:During the experiment,there was no significant difference in fasting body weight between the groups.After modeling,the mice developed mushy stool,and the fecal shape of the moxibustion group was significantly improved at the end of the treatment.Compared with the blank group,the SUA level of the model group was significantly increased(P<0.01),and the serum IL-10 level was significantly decreased(P<0.05).Compared with the model group,the SUA level of the acupuncture group and the moxibustion group both decreased significantly(P<0.05),and no significant difference with the blank group(P>0.05).Compared with the model group,the serum IL-10 level of the moxibustion group was significantly increased(P<0.01),and there was no significant difference with the blank group(P>0.05).The serum IL-10 level of the acupuncture group was significantly lower than the other three groups(P<0.01).There was no significant difference among four groups in the serum TNF-αlevel(P>0.05).Conclusion:Acupuncture and moxibustion can reduce the serum uric acid in hyperuricemia mice and have a therapeutic effect on hyperuricemia,moxibustion can significantly promote the expression of anti-inflammatory factor IL-10,the mechanism of reduce uric acid may be related to improving the intestinal environment.Acupuncture can significantly reduce the expression of anti-inflammatory factor IL-10,and its mechanism of lowering uric acid needs further study.