基于惯性原理的微流控芯片粒子连续分选

Continous Particles Separation of Microfluidic Chip Based on Inertia Principle

根据惯性微流控粒子分选原理,设计并制备了基于收缩-扩张结构的微流控芯片,用以实现不同直径粒子的连续分离,并利用COMSOL软件对通道结构进行了流体速度仿真分析,发现当流体通过收缩通道时,流体速度迅速增大。采用微电子机械系统(MEMS)技术制备了微流控芯片:芯片由聚二甲基硅氧烷(PDMS)通道和玻璃基底两部分组成,利用惯性力和Dean力作用,从而实现粒子有效分选。实验过程中,采用两种不同直径的聚苯乙烯微球作为实验样本,分别在150和300μL/min的高体积流量下将两种粒子的混合溶液注入通道,进行了多次实验,实验结果显示直径5μm的聚苯乙烯微球的分选效率达到93.8%,证明该结构分选效果好、分离效率高。

According to the principle of inertial microfluidic particle separation, a microfluidic chip based on the contraction-expansion structure was designed and fabricated to realize the continuous separation of particles with different diameters. The fluid velocity of the channel structure was simulated and analyzed by COMSOL software. It is found that when the fluid passes through the contraction channel, the fluid velocity increases rapidly. The microfluidic chip was prepared by microelectro-mechanical system(MEMS) technology. The chip is composed of the polydimethylsiloxane(PDMS) channel and the glass substrate. The efficient particle separation was realized by using inertia force and Dean force. During the experiment, two kinds of polystyrene microspheres with different diameters were used as the experimental samples, and the mixed solution of the two particles was injected into the channel at the high volume flow rates of 150 and 300 μL/min, respectively. Several experiments were carried out. The experimental results show that the separation efficiency of the polystyrene microspheres with a diameter of 5 μm reaches 93.8%, proving that the structure has good separation effect and high separation efficiency.