脱矿人牙骨基质粒径大小对垂直骨增量效果的影响

Effect of Size of Demineralized Human Dentin Matrix on Vertical Bone Augmentation

目的:探究不同粒径大小的脱矿人牙骨基质材料对兔颅骨垂直骨增量效果的影响。方法:选择6只新西兰大白兔,每只兔颅骨制备3个9 mm环形裂隙并在其上方固定钛容器,容器内随机植入以下3组:A组脱矿人牙骨基质(0.25~0.5 mm);B组脱矿人牙骨基质(0.5~1 mm);C组空白对照组。植入后12周进行锥形束计算机断层摄影(Cone beam computed tomography,CBCT)检查和组织学观察分析。结果:CBCT结果显示A,B,C三组有明显新骨生成,新骨自基底骨垂直向上生长,脱矿人牙骨基质与兔颅骨结合稳定,垂直骨高度维持良好。组织学观察显示脱矿人牙骨基质和骨组织界面出现不同程度血管化及新生骨。A,B两组新骨形成率无显著差异(P>0.05)。结论:粒径大小对于垂直骨增量的效果没有显著性影响,不同粒径大小的脱矿人牙骨基质在垂直骨增量手术中均显示出良好的骨传导性及骨诱导性。

Objective:To explore the effect of demineralized human dentin matrix materials with different particle sizes on the vertical bone augmentation of rabbit skulls.Methods:Six New Zealand white rabbits were selected.Three 9mm circular fissures were prepared on each rabbit skull and a titanium container was fixed on top of them.The materials were implanted in the container.Group A was implanted with demineralized human dentin matrix with particle size at 0.25-0.5 mm.Group B was implanted with matrix with particle size at 0.5-1 mm.Group C was control group.CBCT examination and histological observation and analysis were performed 12 weeks after implantation.Results:CBCT results showed that three groups had obvious new bone formation.The new bone grew vertically from the basal bone.The demineralized human dentin matrix combined with rabbit skull stably,and the vertical bone height was maintained well.Histological observations showed that the interface between the matrix and bone had varying degrees of vascularization and new bone.There was no difference on the rate of new bone formation between group A and B(P>0.05).Conclusion:Demineralized human dentin matrix had good osteoconductivity and osteoinductivity.The particle size has no significant effect on the vertical bone augmentation.