CYP2E1 mRNA在HBV患者中的表达及意义

Expression and significance of CYP2E1 mRNA in HBV patients

目的评价乙型肝炎(HBV)患者外周血中肝脏细胞色素P450同工酶2E1(CYP2E1)检测的临床意义。方法采用实时定量聚合酶链式反应检测150例HBV患者(实验组)及同期体检健康人群150例(对照组)外周血中CYP2E1 mRNA的表达,比较两组间表达差异并分析其与不同临床指标间的相关性。结果外周血中CYP2E1 mRNA的表达实验组(34.782±2.694)较对照组(6.279±3.131)显著升高,差异有统计学意义(t=84.532,P<0.05),CYP2E1 mRNA的表达在实验组不同临床指标中差异均无统计学意义(P>0.05)。结论CYP2E1在HBV患者外周血中高表达,但其表达水平在临床各指标间差异无统计学意义。

Objective To evaluate the clinical value of clinical significance of detection of cytochrome P450 isozymes E1(CYP2E1)in peripheral blood of patients with hepatitis B.Methods CYP2E1 mRNA was detected in peripheral blood of 150 HBV patients(experimental group)and 150 healthy people(control group)using real-time quantitative PCR,then the relations between CYP2E1 and clinical indexes were analyzed.Results The expressions of CYP2E1 mRNA in peripheral blood in experimental group(34.782±2.694)were significantly higher than that in control group(6.279±3.131),there was statistical difference between two groups,but there were no statistical difference in the expressions of CYP2E1 mRNA in all clinical indexes in experimental group(P>0.05).Conclusion CYP2E1 was highly expressed in peripheral blood of HBV patients,but there was no significant difference in the expression among all clinical indexes.