冠状病毒检测方法的比较

Comparison of blood testing methods for coronavirus

2019年底新型冠状病毒引起的肺炎疫情在世界范围内爆发,如何快速高效检测患者体内的新型冠状病毒是诊断及收治的前提。目前,实验室常用的病毒检测方法主要有第二代测序技术和实时荧光定量PCR技术,但基于CRISPR的检测方法覆盖率不高。本文在此主要列出了基于CRISPR技术开发的核酸检测技术并对比它们的优缺点,以及讨论它们在新型冠状病毒检测中的应用可能性。

At the end of 2019,the pneumonia epidemic caused by the new coronavirus first appeared in China and then broke out worldwide.How to quickly and efficiently detect the new coronavirus in patients is the prerequisite for diagnosis and treatment.At present,the virus detection methods commonly used in the laboratory mainly include the next-generation sequencing technology and real-time PCR technology,but the CRISPR-mediated detection methods are not widely used.Here we mainly list the CRISPR-mediated methods and compare their advantages and disadvantages,and discuss their possible applications in the detection of SARS-CoV-2.