三七药酒中人参皂苷Rg_(1)、人参皂苷Rb_(1)的含量测定

Determination of ginsenoside Rg_(1) and ginsenoside Rb_(1) in Sanqi Wine

目的:建立三七药酒中人参皂苷Rg 1、人参皂苷Rb 1的含量测定方法,为其质量标准提升提供理论基础。方法:采用HPLC梯度洗脱建立三七药酒中人参皂苷Rg 1、人参皂苷Rb 1的含量测定方法。使用Acchrom Unitary C 18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-水(B)为流动相,梯度洗脱(0~20 min,20%A;20~45 min,20%A→46%A;45~55 min,46%A→55%A;50~60 min,55%A;60~61 min,55%A→90%A;61~70 min,90%A),流速1.5 mL·min^(-1),检测波长203 nm,用外标法测定了21个批次的三七药酒中人参皂苷Rg1的含量。结果:人参皂苷Rg 1、人参皂苷Rb 1分别在3.446~344.6μg·mL^(-1)(r=1.0000),6.078~303.9μg·mL^(-1)(0.9999)范围内,线性关系良好,平均加样回收率分别为98.4%(RSD=1.4%,n=6),95.2%(RSD=2.9%,n=6)。结论:建立的方法简单准确,可用于三七药酒中三七的质量控制。

Objective:To establish the determination of the content of ginsenoside Rg 1 and ginsenoside Rb 1 in Sanqi wine,which provided theoretical basis for the improvement of quality standard of Sanqi wine.Methods:HPLC Gradient elution was used to establish the determination method of ginsenoside Rg 1 and ginsenoside Rb 1.The mobile phase was acetonitrile(A)-water(B),gradient elution(0-20 min,20%A;20-45 min,20%A→46%A;45-55 min,46%A→55%A;50-60 min,55%A;60-61 min,55%A→90%A;61-70 min,90%A).Flow rate was 1.5 mL·min^(-1),and detection wavelength was 203 nm.The content of ginsenoside Rg 1 and ginsenoside Rb 1 in 21 batches of Sanqi wine was determined.ResultS:Ginsenoside Rg 1 and ginsenoside Rb 1 showed good linear relationships within the ranges of 3.446-344.6μg·mL^(-1)(r=1.0000)and 6.078-303.9μg·mL^(-1)(0.9999),respectively,whose average recoveries were 98.4%(RSD=1.4%,n=6)and 95.2%(RSD=2.9%,n=6),respectively.Conclusion:The established method is simple and accurate,which can be used to control the quality of Panaxnotoginseng in Sanqi wine.