食管鳞状细胞癌组织中miR-145、miR-765表达变化及意义

Changes and significance of miR-145 and miR-765 expression in esophageal squamous cell carcinoma

目的 探讨食管鳞状细胞癌组织微小RNA(miR)-145、miR-765表达变化及意义。方法 选择食管鳞状细胞癌患者80例,应用实时荧光定量聚合酶链反应(qRT-PCR)法检测癌组织及癌旁组织中miR-145、miR-765表达;应用免疫组化法检测组织中增殖细胞核抗原,计算阳性细胞百分数作为增殖指数(PI);应用Td T介导的脱氧尿苷酸缺口末端标记(TUNEL)法测算凋亡指数(AI);分析食管鳞状细胞癌组织中miR-145、miR-765表达与患者临床病理特征的关系;随访36个月,观察不同miR-145、miR-765表达患者生存情况;分析患者死亡的危险因素。结果食管鳞状细胞癌组织中miR-145表达、AI低于癌旁组织,miR-765表达、PI高于癌旁组织(P均<0.05)。Pearson相关分析显示,食管鳞状细胞癌组织中miR-145表达与PI呈负相关(r=-0.522,P <0.05),与AI呈正相关(r=0.578,P <0.05);miR-765表达与PI呈正相关(r=0.602,P <0.05),与AI呈负相关(r=-0.511,P <0.05)。miR-145、miR-765表达与组织分化程度、TNM分期有关(P均<0.05)。经Log-rank检验发现,miR-145高表达组生存率高于miR-145低表达组,miR-765低表达组生存率高于miR-765高表达组(P <0.05)。Cox分析结果显示,低分化、TNM分期Ⅲ期、miR-145低表达、miR-765高表达是食管鳞状细胞癌患者死亡的危险因素(P均<0.05)。结论 食管鳞状细胞癌组织中miR-765异常高表达、miR-145异常低表达,二者均与癌细胞增殖有关;检测二者表达有助于食管鳞状细胞癌病情判断及预后评估。

Objective To investigate the expression changes and significance of microRNA(miR)-145 and miR-765 in the esophageal squamous cell carcinoma tissues.MethodsEighty patients with esophageal squamous cell carcinoma were selected and the expression levels of miR-145 and miR-765 in the cancer and adjacent tissues were detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Immunohistochemical staining was used to detect tissue proliferative nuclear antigen and the percentage of positive cells was calculated as proliferation index(PI).Apoptosis index(AI)was detected by TdT-mediated deoxyuridine notch end labeling(TUNEL).The relationships between the miR-145 and miR-765 expression in esophageal squamous cell carcinoma tissues and the clinicopathological characteristics of patients were analyzed.At 36 months after follow-up,the survival of patients with different miR-145 and miR-765 expression were observed.The risk factors for death of patient were analyzed.Results The miR-145 expression and AI in the esophageal squamous cell carcinoma tissues were significantly lower than those in the paracancerous tissues,and the miR-765 expression level and PI were significantly higher than those in the paracancerous tissues(all P<0.05).Pearson correlation analysis showed that the miR-145 expression was negatively correlated with PI(r=-0.522,P=0.023),and positively correlated with AI(r=0.578,P=0.011).The miR-765 expression level was positively correlated with PI(r=0.602,P=0.000),and negatively correlated with AI(r=-0.511,P=0.017).The miR-145 and miR-765 expression levels were related to the tissue differentiation degree and TNM stage(all P<0.05).Log-rank test showed that the survival rate of the high miR-145 expression group was significantly better than that of the low miR-145 expression group,and the survival rate of the low miR-765 expression group was significantly better than that of the high miR-765 expression group(both P<0.05).Results of Cox analysis showed that low differentiation,TNM stage III,low miR-145 expression and high miR-765 expression were risk factors for death in patients with esophageal squamous cell carcinoma(all P<0.05).Conclusions There is abnormal high expression of miR-765,and low expression of miR-145 in esophageal squamous cell carcinoma tissues,both of which are associated with cancer cell proliferation.Detection of their expression is helpful for the diagnosis and survival evaluation of esophageal squamous cell carcinoma.