经典瞬时受体电位通道3基因多态性与原发性高血压患者微量白蛋白尿的关系

Association between classical transient receptor potential channel 3 gene polymorphism and MAU in patients with essential hypertension

目的探讨经典瞬时受体电位通道3(TRPC3)基因多态性与原发性高血压患者微量白蛋白尿(MAU)的关系。方法选取原发性高血压患者共449例,根据24 h尿微量白蛋白水平分为高血压组(EH组)378例和高血压合并MAU组(EH+MAU组)71例。利用PCR扩增、MassArray技术对HapMap数据库中筛选TRPC3的5个SNP位点进行基因分型。结果 (1)两组间年龄、体质量指数、甘油三酯、24 h平均收缩压及舒张压比较差异有统计学意义(P <0.05)。(2)两组rs953691位点基因型分布、等位基因及显性模型(GG/GT+TT)比较差异有统计学意义(P <0.05)。(3)logistic回归分析显示:甘油三酯、24 h平均收缩压(≥150 mmHg)和rs953691位点GG基因型是原发性高血压患者MAU的危险因素。结论 TRPC3基因rs953691位点的GG基因型可能增加高血压患者MAU的发生风险。

Objective To investigate the association between classical transient receptor potential channel3(TRPC3)gene polymorphism and microalbuminuria(MAU)in patients with essential hypertension. Methods A total of 449 patients were recruited and the patients were assigned to two groups:Hypertension group(EH group,n = 378)and hypertension with MAU group(EH+MAU group,n = 71)according to microalbuminuria in24 h. PCR amplification,Mass Array were used for genotyping the single nucleotide polymorphism(SNP)sites of the TRPC3 gene. Results There were significant differences in age,body mass index,triglycerides,24 h mean systolic blood pressure,and 24 h mean diastolic blood pressure between the two groups(P < 0.05). There were significant differences in the genotype,allele and dominant model(GG/GT + TT)at rs953691(P < 0.05). Logistic regression analysis showed triglycerides,24 h mean systolic blood pressure(≥ 150 mmHg),and GG genotype at rs953691 were risk factors for MAU in patients with essential hypertension. Conclusion The GG genotype at rs953691 in the TRPC3 gene may increase the risk of MAU in hypertensive patients.