红曲发酵藜麦种子液培养及固态发酵条件优化

Optimization of Culture and Solid-State Fermentation Conditions for Quinoa Seed Liquid Fermented by Monascus

为探究红曲-藜麦固态发酵适生性,提高藜麦的食用价值,以藜麦为发酵基质,益生菌-红曲菌为发酵菌种,探究红曲发酵藜麦种子液培养条件及固态发酵条件,分别对种子液的培养条件进行单因素分析,确定因素水平,以菌体干重为评定指标进行正交优化试验,判断最优种子液培养条件,基于最优种子液培养条件对藜麦进行固态发酵,分别对固态发酵培养条件进行单因素试验,以色价为评定指标进行正交试验,以期获得最优固态发酵条件。结果表明,当接种量为20%、初始pH值为5、装液量为30 mL/250 mL时种子液菌体生长情况最优,菌体重量达57.13 g/L,当蒸料时间为50 min,接种量为15%、料液比为1∶1、发酵时间为10 d、发酵温度为31℃时,红曲-藜麦固态发酵菌体色价最优,达11.12 U/g。

In order to explore the suitability of solid-state fermentation of quinoa-Monascus,increase the edible value of quinoa,quinoa is used as the fermentation substrate,probiotics-Monascus is selected as the fermentation strain.Single factor experiment is carried out for the culture conditions of seed liquid,the factors and levels are determined,and the orthogonal experiment is carried out with the dry weight of bacteria as the evaluation index,the optimal seed culture conditions are identified.The solid-state fermentation of quinoa is carried out based on the optimal culture conditions of seed liquid.The single factor test is carried out on the culture conditions of solid-state fermentation,and the orthogonal test is carried out with color value as the evaluation index,in order to obtain the optimal conditions of solid-state fermentation.The results show that when the inoculation amount is 30%,the initial pH value is 5,and the liquid amount is 30 mL/250 mL,the growth of seed liquid bacteria is the best,and the bacterial weight reaches 57.13 g/L.When the steaming time is 50 min,the inoculation amount is 15%,the solid-liquid ratio is 1∶1,the fermentation time is 10 d and fermentation temperature is 31℃,the color value of M onascus-quinoa solid-state fermentation bacteria is the best,reaching 11.12 U/g.