UV和PMA引起的炎症敏感性皮肤的体外功效评估方法研究

Research on in vitro efficacy evaluation methods for sensitive skin caused by UV and PMA

通过紫外(UV)辐照和PMA(PHORBOL 12-MYRISTATE 13-ACETATE)2种不同压力源刺激人原代角质形成细胞释放炎症因子,并利用酶联免疫吸附测定方法测定炎症因子TNF-α和IL-1α释放量以确定该炎症模型的有效性。结果表明,在不同的压力源下,炎症因子相对含量均有不同程度的升高,因此该炎症模型可进一步使用功效成分进行验证。进一步使用功效成分进行验证,结果表明,该炎症模型在验证功效成分对炎症因子的影响与人体数据一致,因此该炎症模型可以作为开发护理炎症敏感性皮肤的功效成分提供实验数据支持。

Sensitive skin has become an increasingly concerned skin problem in the global skin care product market.Sensitive skin caused by inflammation is on the increase.Therefore,the development of skin care products for inflammation-sensitive skin has become an important way to solve this problem.In this work,an in vitro research platform for cosmetic raw materials was established for inflammatory sensitive skin caused by ultraviolet(UV)radiation and PMA(PHORBOL 12-MYRISTATE 13-ACETATE),which might provide support for the development of functional ingredients for sensitive skin care.The inflammation model was established with human epidermal primary keratinocytes.UV radiation and PMA were used to stimulate human primary keratinocytes.The release of inflammatory factors TNF-α and IL-1α was measured by enzyme-linked immunosorbent assay method to determine the effectiveness of the inflammation model.The results show that,the relative content of inflammatory factors is increased to varying degrees in both cases of UV radiation and PMA,so the inflammation model can be further verified with functional components.Dexamethasone was used as a positive reference material,and dipotassium glycyrrhizinate,bisabolol,and portulaca were used as active materials to verify the effectiveness of the model.The results show that dipotassium glycyrrhizinate and bisabolol can inhibit UV and PMA-mediated production of TNF-α and IL-1α to varying degrees,thereby reducing the occurrence of skin sensitivity.The inflammation model is good in verifying the effects of functional components on inflammatory factors.Therefore,the inflammation model can provide experimental data support for the development of functional components for care of sensitive skin.