血清miR-210和miR-375在子宫颈癌早期诊断与术后监测的应用研究

Application of Serum miR-210 and miR-375 in Early Diagnosis and Postoperative Monitoring of Cervical Cancer

目的:分析血清微小RNA-210(miR-210)和miR-375在子宫颈癌早期诊断与术后监测的应用效果。方法:选择2019年1月-2020年7月本院收治的30例子宫颈癌患者为子宫颈癌组;20例宫颈上皮内瘤变患者为宫颈上皮瘤变组;20例健康者为健康对照组。所有入选者均检测肿瘤标志物水平,记录三组血清miR-210、miR-375、SCC水平以及在子宫颈癌患者手术前后中的表达变化情况,分析其在子宫颈癌早期诊断和术后监测的临床意义。结果:子宫颈癌组的miR-210、SCC均高于宫颈上皮内瘤变组和健康对照组,miR-375低于宫颈上皮内瘤变组和健康对照组,且宫颈上皮内瘤变组的miR-210、SCC均高于健康对照组,miR-375低于健康对照组,差异均有统计学意义(P<0.05)。术后3个月,子宫颈癌患者miR-210、SCC均低于术前,miR-375高于术前,差异均有统计学意义(P<0.05)。ROC曲线结果显示,血清miR-210、miR-375、SCC单独与联合用于诊断子宫颈癌的曲线下面积分别为:0.710、0.816、0.811、0.843,具有一定的诊断性能。结论:子宫颈癌早期诊断与术后监测中miR-210、miR-375、SCC具有重要意义,联合诊断应用价值较高,能够反映患者病情。

Objective:To analyze the effect of serum microRNA-210(miR-210)and miR-375 in the early diagnosis and postoperative monitoring of cervical cancer.Method:A total of 30 patients with cervical cancer admitted to our hospital from January 2019 to July 2020 were selected as cervical cancer group;20 patients with cervical intraepithelial neoplasia were included in the cervical intraepithelial neoplasia group;20 healthy subjects were selected as the healthy control group.The levels of tumor markers were detected in all the selected patients,and the levels of miR-210,miR-375 and SCC in serum of the three groups were recorded,as well as the expression changes in cervical cancer patients before and after surgery,and their clinical significance in the early diagnosis and postoperative monitoring of cervical cancer was analyzed.Result:The miR-210 and SCC of the cervical cancer group were higher than those of the cervical intraepithelial neoplasia group and healthy control group,and the miR-375 was lower than that of the cervical intraepithelial neoplasia group and healthy control group,and the miR-210 and SCC of the cervical intraepithelial neoplasia group were higher than those of the healthy control group,miR-375 was lower than the healthy control group,the differences were statistically significant(P<0.05).3 months after surgery,miR-210 and SCC of cervical cancer patients were lower than those before surgery,and miR-375 was higher than that before surgery,the differences were statistically significant(P<0.05).The ROC curve results showed that the areas under the curve of serum miR-210,miR-375 and SCC for the diagnosis of cervical cancer alone and in combination were 0.710,0.816,0.811 and 0.843,respectively,showing certain diagnostic performance.Conclusion:miR-210,miR-375,and SCC are of great significance in the early diagnosis and postoperative monitoring of cervical cancer,the sensitivity,specificity,and specificity of combined diagnosis are all high,which can reflect the patient’s condition.