槲皮素对镉致大鼠肾脏损伤的保护作用

Cadmium Induced Kidney Injury in Rats and the Protective Effect of Quercetin

为探讨镉对大鼠肾脏损伤以及槲皮素对其的保护作用。将24只雄性SD大鼠随机分为4组,每组6只。每天分别按如下处理:对照组(生理盐水灌服)、镉处理组(按体重1 mg·kg^(-1)腹腔注射CdCl_(2))、槲皮素组(按体重100 mg·kg^(-1)灌服)、镉+槲皮素组(按体重1 mg·kg^(-1)腹腔注射CdCl_(2),同时按体重100 mg·kg^(-1)灌服槲皮素)。试验周期28 d,而后处死大鼠。收集大鼠血清并检测血清中尿酸(UA)、肌酐(CRE)和尿素氮(BUN)含量;收集肾脏并制备组织匀浆,检测组织匀浆中还原型谷胱甘肽(GSH)、丙二醛(MDA)的含量、超氧化物歧化酶(SOD)以及过氧化氢酶(CAT)活性;取大鼠肾脏组织制备组织切片,并观察组织病理变化。结果表明:镉可显著提高血清中UA、CRE的含量(P<0.05),以及肾组织中GSH和MDA的含量,显著降低肾脏中SOD和CAT的活性。与镉组相比,槲皮素可以显著降低血清中UA、CRE含量,显著降低肾脏组织中GSH和MDA的含量,显著升高肾脏中CAT的活性,槲皮素可升高SOD活性但差异不显著。肾脏的组织学切片表明,镉会引起肾皮质中毛细血管充血;细胞有不同程度的坏死等变化,而镉+槲皮素可以明显减轻损伤。由此可知,镉致大鼠的肾组织产生脂质过氧化损伤和组织损伤,而槲皮素对镉导致的这种损伤具有一定的保护效应。

To investigate the renal injury of cadmium in rats and the protective effect of quercetin,twenty-four male SD rats were randomly divided into 4 groups with 6 rats in each group.Each day was treated as follows:control group(intragastric administration of normal saline),cadmium treatment group(1 mg·kg^(-1) intraperitoneal injection of CdCl_(2)),quercetin group(100 mg·kg^(-1) gavage of quercetin),and cadmium+quercetin group(1 mg·kg^(-1) intraperitoneal injection of CdCl_(2) and 100 mg·kg^(-1) gavage of quercetin).The test period was 28 days,and then the rats were killed.The serum of rats was collected and the contents of uric acid(UA),creatinine(CRE)and urea nitrogen(BUN)were detected;The contents of reduced glutathione(GSH),malondialdehyde(MDA)and the activities of superoxide dismutase(SOD)and catalase(CAT)were detected;The renal tissue of rats was taken to prepare tissue sections,and the histopathological changes were observed.The results showed that cadmium could significantly increased the contents of UA and CRE in serum(P<0.05),GSH and MDA in renal tissue,and significantly reduced the activities of SOD and CAT in kidney.Compared with cadmium group,quercetin could significantly reduced the contents of UA and CRE in serum,significantly reduced the contents of GSH and MDA in kidney tissue,and significantly increased the activity of CAT in kidney,beside quercetin could increase SOD activity,but the difference was not significant.Histological sections of kidney showed that cadmium could cause capillary congestion in renal cortex;The cells had different degrees of necrosis,and cadmium+quercetin could significantly reduced the damage.Therefore,cadmium induced lipid peroxidation injury and tissue injury in rat kidney,and quercetin had a certain protective effect on the injury caused by cadmium.