基于密度调节的液液微萃取结合高效液相色谱测定药茶、陈醋、汾酒中的双酚A

Determination of bisphenol A content in medicinal tea, vinegar, and Fenchiew using density-adjusted liquid-liquid microextraction combined with HPLC

[目的]本文旨在建立一种基于密度调节的液液微萃取-高效液相色谱-荧光检测器检测药茶、陈醋、汾酒中双酚A的分析方法,快速高效地完成山西特色食品样品的分析。[方法]选用环境友好的愈创木酚作为萃取剂,调节密度实现萃取剂的"上浮-下沉",促进萃取剂与样品溶液的充分接触,实现快速高效的萃取。萃取完成后,收集下层的萃取剂,在激发(发射)波长282(313)nm处用高灵敏度的荧光检测器进行定量分析。[结果]通过优化萃取剂体积、盐种类、加盐用量、加水体积和pH等影响因素,得到了最佳的萃取条件。双酚A在0.04~16μg·mL^(-1)范围内线性关系良好,检出限为0.01μg·mL^(-1),回收率为73.0%~87.5%。[结论]通过调节密度控制萃取剂的"上浮-下沉"的前处理方法可以应用于药茶、陈醋、汾酒中双酚A的提取,是一种适用于食品样品的环境友好、快速高效的有害物质的前处理方法。

[Objective]The study aimed to establish a method to detect bisphenol A in medicinal tea,vinegar,and Fenchiew based on density-adjusted liquid-liquid microextraction-HPLC-FLD to achieve fast and efficient analysis of traditional Shanxi foods.[Methods]In this study,the environment-friendly guaiacol was used as the extractant,and its density was adjusted to achieve the"floating-sinking"of the extractant,which promoted the full contact between the extractant and the sample solution and fulfilled rapid and efficient extraction.When the extraction was completed,the extractant in the lower layer was collected and quantitatively analyzed by a sensitive fluorescence detector at the excitation(emission)wavelength of 282(313)nm.[Results]The optimal extraction conditions were obtained by adjusting the volume of the extractant,the type of salt,the amount of salt added,the volume of water added,pH value and other influencing factors.Bisphenol A showed a good linear relationship in the concentration range of 0.04~16μg·mL^(-1),and the limit of detection was 0.01μg·mL^(-1),and the recovery rate was73.0%~87.5%.[Conclusion]The pretreatment method based on density-adjusted“floating-sinking”process can be successfully applied to the detection of bisphenol A in medicinal tea,vinegar,and Fenchiew.It is an environmentally friendly,fast and efficient pretreatment method of the harmful substances in food samples.