灯盏花化妆品原料的抗氧化活性及质量控制研究

Antioxidant activity analysis and quality control of cosmetic ingredients from Erigeron breviscapus

对灯盏花化妆品原料(EBCI)的抗氧化活性进行研究,同时建立其中两种指标成分的含量测定方法。采用DPPH、ABTS、·OH清除法考察EBCI的自由基清除活性,同时用H_(2)O_(2)诱导HHF-1细胞氧化损伤模型考察EBCI对细胞存活率和细胞中谷胱甘肽(GSH)含量的影响;建立了HPLC法同时测定EBCI中野黄芩苷和4,5-二-O-咖啡酰奎宁酸的含量测定方法。结果显示EBCI具有DPPH、ABTS和·OH自由基清除能力,IC_(50)分别为46.93、91.36和89.37μg/mL,在4、20μg/mL质量浓度下能显著提高氧化损伤HFF-1细胞的存活率且能显著提高细胞中GSH含量。野黄芩苷和4,5-二-O-咖啡酰奎宁酸分别在0.0093~0.3722、0.0085~0.3409 mg/mL范围内呈现良好线性关系,加样回收率分别为103.47%、103.38%(RSD均小于2%)。结果表明EBCI具有抗氧化作用;所建立的含量测定方法简便、稳定、准确可靠,可用于EBCI质量控制。

Erigeron breviscapus(vant.)Hand.Mazz.is one of the most important traditional Chinese medicinal plants for cardiopathy.Erigeron breviscapus mainly contains flavonoids and caffeic acids,which have obvious antioxidant activities.To study the possibility of developing Erigeron breviscapus as a cosmetics material,the antioxidant activity of Erigeron breviscapus cosmetic ingredients(EBCI)was studied and a method for simultaneous determination of two index compounds therein was established.The scavenging activities of EBCI against DPPH,ABTS and·OH radicals were evaluated by in vitro method.The effects of EBCI on cell viability and glutathione(GSH)content were investigated by using H_(2)O_(2)-induced damage in HHF-1 cells.HPLC method for simultaneous determination of scutellarin and 4,5-dicaffeoylquinic acid in EBCI was established.The results show that EBCI have scavenging activities against DPPH,ABTS and·OH radicals,with IC_(50) values being 46.93,91.36 and 89.37μg/mL,respectively.The cell viability of H_(2)O_(2)-induced injury in HFF-1 cells and the GSH content in the cells could be significantly increased at the concentrations of 4 and 20μg/mL.4,5-Dicaffeoylquinic acid and scutellarin showed good linearity in the linear ranges of 0.0085-0.3409 mg/mL and 0.0093-0.3722 mg/mL,respectively.Average recoveries of scutellarin and 4,5-dicaffeoylquinic acid were 103.47%and 103.38%(RSD<2%),respectively.This method is accurate,simple and well reproducible,and can be used for the quality control of EBCI.It shows the antioxidant activity of EBCI in this study.