液质联用法测定甘草组方化妆品中4种标志性成分

Determination of four characteristic components in Glycyrrhiza uralensis based cosmetics by LC-MS method

建立液相色谱-质谱法测定宣称含有甘草成分的植物组方化妆品,以饱和氯化钠分散样品,乙腈超声提取,以0.1%甲酸水-乙腈作为流动相梯度洗脱,流速为0.4 mL/min,多反应监测(MRM)模式检测。4种成分在各自范围内线性关系良好,平均加样回收率85%~104%,RSD为1.1%~10.0%,方法检出限在1~15 ng/g之间;12批样品中检出符合率为41.6%。目前市售的植物组方中宣称甘草成分化妆品中,甘草成分检出率和检出含量较低,商家存在一定的概念营销,相关部门应加强监管。

A LC-MS method was established for determination of botanical cosmetics claimed to contain ingredients from Glycyrrhiza uralensis.The sample was dispersed by saturated solution of sodium chloride and was ultrasonically extracted with acetonitrile.0.1%formic acid water-acetonitrile was used as the mobile phase for gradient elution at a flow rate of 0.4 mL/min.Multiple reaction monitoring(MRM)mode was used for detection.The four components had good linear relationship within their respective range.The average recovery rate was 85%-104%,the RSD was 1.1%-10.0%,and the detection limit of the method was between 1 and 15 ng/g;12 batches of samples were tested and the coincidence rate thereof was 41.6%.The test results indicate that the presence and the amount of ingredients from Glycyrrhiza uralensis are actually relatively low in as-claimed cosmetics.In other words,there are conceptual marketing and false publicity and thus relevant supervision should be strengthened.