纳米纤维固定化鸡肝酯酶及其在西维因检测中的应用

Immobilization of chicken liver esterase by nanofibers and its application in detection of carbaryl

动物酯酶可作为酶抑制法农残检测的候选酶源,但需要固定化载体提高其对农药响应的敏感性。通过静电纺丝普鲁兰多糖(P)纳米纤维固定化鸡肝酯酶(E),并对其结构特征和对西维因农药抑制响应进行研究。结果发现:高(4P3E)和低(4P1E)两种不同鸡肝酯酶添加量的P复合纳米纤维平均直径分别为75.62和70.39 nm。扫描电镜能谱分析表明,鸡肝酯酶在P复合纳米纤维上分布均匀。不同浓度(0.5~10.0 mg/L)西维因的农药抑制响应结果表明,西维因对4P1E的酶活抑制率小于4P3E,但4P1E的检测下限(0.81 mg/L)优于4P3E(1.10 mg/L),可考虑进一步进行产品优化和效果评价,从而应用于实际的西维因检测工作中。

Animal esterase can be used as a candidate enzyme source for the detection of pesticide residues by enzyme inhibition method,but the immobilized carrier is needed to improve its sensitivity to pesticides.Chicken liver esterase(E)was immobilized by electrospun pullulan(P)nanofiber,and its structural characteristics and inhibition response to carbaryl pesticide was studied.The results showed that the average diameter of P composite nanofiber with two different kinds of chicken liver esterase addition(High:4P3E and low:4P1E)was 75.62 nm and 70.39 nm,respectively.Scanning electron microscope-energy spectrum analysis presented that chicken liver esterase was distributed evenly in P composite nanofiber.The inhibition response of carbaryl with different concentrations(0.5~10 mg/L)showed that the inhibition rate of carbaryl to 4P1E was less than 4P3E,but the detection limit of 4P1E(0.81 mg/L)was higher than 4P3E(1.10 mg/L),which should be further considered for product optimization and effect evaluation to the actual carbaryl detection.