利用QTL-seq定位萝卜肉质根根形指数QTL

Identification of quantitative trait loci controlling radish root index by QTL-seq

为检测萝卜肉质根根形指数相关的数量性状遗传位点(QTL),挖掘调控萝卜肉质根根形的关键基因,以肉质根根形指数差异显著的小型扁圆萝卜LLYH和大型长白萝卜CLA为亲本,杂交构建了F_(2)分离群体,利用QTL-seq方法对肉质根根形指数性状进行了QTL定位分析。一共检测到4个QTL位点,其中两个主效QTL位点rs2.1和rs2.2,分别位于萝卜R2号染色体21.66~26.03 Mb和30.79~36.56 Mb区域,两个微效QTL位点rs4.1和rs6.1,分别位于萝卜R4号染色体39.04~40.43 Mb和R6号染色体11.53~13.40 Mb。研究结果为开发与萝卜肉质根根形指数连锁的分子标记奠定了基础,同时为挖掘肉质根根形指数调控基因提供了重要参考。

In order to detect QTL loci and find the key genes related to radish root shape index,an F_(2) population was constructed by crossing the small-sized oblate spheroidal radish LLYH and big-sized long white radish CLA which had significant differences in root shape index.QTL mapping for root shape index traits was conducted using QTL-Seq method.Four QTL loci were detected,of which two major QTL loci rs2.1 and rs2.2 were located in the 21.66-26.03 Mb and 30.79-36.56 Mb regions on chromosome R2,respectively;two minor QTL loci rs4.1 and rs6.1 were located on chromosome R439.04-40.43 Mb and R611.53-13.40 Mb.These results laid a foundation for the development of molecular markers linked to root shape index and provided an important reference for fine mapping the regulatory genes of the root shape index.