摘要
以4-(2-吡啶基)苯甲醛、IrCl_(3).3H_(2)O为原料,通过还原反应和亲核取代等反应设计合成了一种铱配合物探针IrL,利用氢谱、碳谱和质谱对探针进行了表征。结果表明探针IrL能够专一性识别组氨酸及富含组氨酸的蛋白质并发出明亮的绿光,检测限低至0.132μmol/L。细胞实验结果表明探针IrL具有较低的细胞毒性,主要靶向在固定细胞内的细胞核区域。此实验结果为后期设计组氨酸探针奠定了实验基础。
A new iridiunm(Ⅲ)complex probe(IrL)was designed and synthesized from 4-(2-pyridinyl)benzaldehyde and IrCl_(3)·3 H_(2)O through reduction reaction and nucleophilic substitution reaction.The probe was characterized by NMR spectroscopy and mass spectrometry.The experimental results demonstrated that probe IrL could specifically detect histidine and histidinerich proteins with an obvious fluorescence enhancement and the detection limit was 0.132μmol/L.The cell imaging results exhibited that probe IrL possesses low cytotoxicity and lights up the nuclear regions of the fixed HepG2 cells,which provides the experimental basis for the further design of histidine probes.
作者
许丙嵩
陈浩
胡磊
王慧
XU Bingsong;CHEN Hao;HU Lei;WANG Hui(School of pharmacy,Wannan Medical College,Wuhu Anhui 241002,China)
出处
《阜阳师范大学学报(自然科学版)》
2021年第4期24-30,共7页
Journal of Fuyang Normal University:Natural Science
基金
安徽省自然科学基金资助项目(1908085QB50)
皖南医学院青年骨干人才资助项目(wyqnyx202006)
皖南医学院大学生科研资助项目(WK2020S39)
皖南医学院校中青年科研基金项目(WK202012)。
关键词
组氨酸
铱配合物
荧光探针
细胞成像
histidine
iridium(Ⅲ)complex
fluorescent probe
cell imaging